ECHİNOCOCCUS GRANULOSUS KAYNAKLI KİST HİDATİK SIVISININ METABOLOMİK ANALİZİ

Abstract

Nenni, M. Metabolomical Analysis of Hydatic Cyst Fluid from Echinococcus granulosus, Hacettepe University Graduate School of Health Sciences One Health Master Thesis, Ankara, 2024. The zoonotic disease called cystic echinococcosis (CE) is caused by the Echinococcus granulosus sensu lato species complex. The disease can be transmitted to people and livestock by ingesting fecal matter. CE continues to have a substantial presence in China, Asia, America, Africa, and the Mediterranean. Approximately one million individuals worldwide are affected, with significant consequences for veterinary and public health, as well as social and economic matters. The formation of cysts has been attributed to the gradual progression of the condition. The prevalence of these cysts is highest in the lung and liver. There is uncertainty about the exact path these cysts will take. This thesis study aimed to investigate the mechanisms of the immunological effects of liver and lung CE cysts in cattle by analyzing their chemical profiles using metabolomics and multivariate statistical analysis. Researchers employed non-targeted metabolomic methods using quadrupole-time-of-flight liquid chromatography/mass spectrometry (LC-QTOF-MS) to distinguish between liver and lung CE cysts. The XCMS software was performed the data processing required for extracting the peaks from intricate chromatograms. The plots of principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA), obtained by various statistical methods, revealed the interactions of metabolites within and between different groups. The liver and lung hydatid cysts include metabolites such as glutathione, prostaglandin, folic acid, and cortisol, which have a role in many immunological responses. Nevertheless, the proportions of these metabolites vary between the liver and lung cysts. Determining the immunological effects and host immune responses that may be caused by differences in the metabolomic profiles of liver and lung cysts identified in this thesis will help further research aimed at elucidating the biology of the cyst and developing specific therapeutic approaches.