Hacettepe Üniversitesi Açık Erişim Sistemi (HÜAES)
- HÜAES, Hacettepe Üniversitesi bünyesinde üretilen kitap, makale, tez, bildiri, rapor gibi tüm akademik kaynakları uluslararası standartlarda dijital ortamda depolar, etkisini artırmak için telif haklarına uygun olarak Açık Erişime sunar.
Communities in DSpace
Select a community to browse its collections.
- Ankara State Conservatory
- Directorates
- Faculty of Dentistry
- Faculty of Pharmacy
- Faculty of Letters
Recent Submissions
Türkiye'de Tıbbi Çay Olarak Kullanılan Lamiaceae Familyasından Çeşitli Bitkilerin Fenolik Asit Miktarlarının ve Biyolojik Etkilerinin Araştırılması
(Sağlık Bilimleri Enstitüsü, 2027-01-01) Zeynep SOLAKLAR; Farmakognozi
Many plants in the Lamiaceae family are widely consumed as herbal teas due to their aromatic properties and therapeutic potential. This study aimed to determine the content of rosmarinic acid and chlorogenic acid, two phenolic acids of high medicinal importance, using High-Performance Liquid Chromatography (HPLC) in aqueous infusions and 80% (v/v) ethanol extracts of twelve Lamiaceae species naturally growing or cultivated in Türkiye. Additionally, the antidiabetic potential of these extracts was evaluated using α-glucosidase and dipeptidyl peptidase-4 (DPP-4) inhibition assays, while their depigmenting potential was assessed through tyrosinase enzyme inhibition. According to the results, the highest rosmarinic acid content was detected in the 80% ethanol extract of Melissa officinalis (29.808 mg/g dry weight), whereas the highest chlorogenic acid content was determined in the 80% ethanol extract of Lavandula × intermedia (1.573 mg/g dry weight). In biological activity analyses, the aqueous infusion extract of Mentha × piperita exhibited the highest inhibition of the DPP-4 enzyme (44.379%), while the Melissa officinalis aqueous infusion demonstrated the highest inhibition of the α- glucosidase enzyme (>99% in all tested concentrations), displaying the strongest antidiabetic activity. Regarding tyrosinase enzyme inhibition, the highest activity was observed in the Salvia officinalis aqueous infusion extract, with an inhibition rate of 64.19%. The findings regarding high phenolic content and enzyme inhibition highlight the potential of Lamiaceae members as strategic natural raw materials and suggest that they should be supported by further research.
OSTEOMYELİT GÖRÜNTÜLEME VE TEDAVİSİNDE KULLANILMAK ÜZERE RADYOİŞARETLİ FUSİDİK ASİT VE LEVOFLOKSASİN İÇEREN LİPOZOM FORMÜLASYONLARININ GELİŞTİRİLMESİ VE ETKİNLİĞİNİN İZLENMESİ
(Sağlık Bilimleri Enstitüsü, 2026) Ecz. Asuman TAŞ; Radyofarmasi
ABSTRACT
Taş A., Development and Evaluation of Radiolabeled Liposomal Formulations
Containing Fusidic Acid and Levofloxacin for Imaging and Treatment of
Osteomyelitis, Hacettepe University, Graduate School of Health Sciences,
Radiopharmacy Doctoral Programme, Doctoral Thesis, Ankara, 2026. Osteomyelitis
is a disease characterized by inflammation and swelling of bone tissue. Compared to
other infection models, significant challenges are encountered in the diagnosis and
effective treatment of osteomyelitis. Laboratory tests, microbiological analyses, and
radiological imaging techniques are used in the diagnosis. The administration of
antibiotics using drug delivery systems enables therapeutic efficacy at very low
concentrations while significantly reducing side effects and allowing the combined
delivery of two different drugs. Liposomal systems, one of the most widely used
delivery platforms, offer advantages such as enhanced bioavailability of drugs, the
ability to specifically target tissues, and being biocompatible and non-toxic. Within
the scope of this study, PEGylated, nanosized theranostic liposomes actively targeted
to bone via DPPE-Asp conjugation and radiolabeled with Gallium-68 (⁶⁸Ga) through
DOTA-Bn-DSPE synthesis were formulated for the diagnosis and treatment of
osteomyelitis. These liposomes co-encapsulated fusidic acid and levofloxacin as a
combined drug therapy, and their characterization, in vitro stability, in vitro
antimicrobial activity, and in vivo efficacy were evaluated. Asp-Lipo/LEV/FA
liposomes were developed in the nanoscale range (220.31 ± 2.40 nm) and showed
encapsulation efficiencies (8.62 ± 1.27% for levofloxacin and 31.20 ± 2.04% for fusidic
acid). The highest ⁶⁸Ga radiolabeling efficiency for Asp-Lipo/LEV/FA was obtained at
60 °C, pH 4.5, within 5 minutes. All liposomal formulations remained stable at 4 °C
for 30 days. Asp-Lipo/LEV/FA demonstrated significantly lower minimum inhibitory
concentration (MIC) values against both E. coli and S. aureus strains compared to
Lipo/LEV/FA and single-drug-loaded liposomes. 68Ga-labeled Asp-Lipo/LEV/FA
showed greater uptake than 68Ga-labeled Lipo/LEV/FA formulations and was more
effective in terms of treatment efficacy than the LEV/FA solution due to MicroPET/CT
imaging and biodistribution studies. In conclusion, both 68Ga-labeled Asp Lipo/LEV/FA and 68Ga-labeled Lipo/LEV/FA have potential for use in the imaging and
treatment of osteomyelitis.
Keywords: Osteomyelitis, Bone targeting, Fusidic acid, Levofloxacin, 68Ga Radiolabeling, Theranostic liposomal systems.
Supported by: H.U.BAP Project No: 19200 and
H.U.BAP Project No: 21978.
Plantago major subsp. intermedia (Gilib.) Lange ve Plantago scabra Moench.'un antienflamatuvar ve yara iyi edici etkilerinden sorumlu ilaç adayı bileşiklerin araştırılması
(Sağlık Bilimleri Enstitüsü, 2026-03-05) Hilal Bacanak; Farmakognozi
The genus Plantago (Plantaginaceae) is represented by approximately 275 species worldwide, and in the flora of Türkiye, there are a total of 23 species, 2 of which are endemic. Plantago species are traditionally used in the treatment of various diseases such as wounds, burns, hemorrhoids, pain, fever, swelling, insect bites, respiratory diseases, stomach ulcers, and diabetes. In this thesis study, 80% ethanol extracts of the aerial parts of Plantago major subsp. intermedia and Plantago scabra were prepared. Phytochemical and biological activity studies were carried out on these extracts. The structures of 18 of the compounds isolated as a result of the phytochemical studies were elucidated using advanced spectroscopic methods. Five of these compounds were identified as iridoid glucosides (aucubin, 10-hydroxymajoroside, 10-acetoxymajoroside, plantarenaloside, and feruloyl gardoside), two as phenylethanoid glycosides (acteoside and martynoside), one as a phenol glucoside (isotachioside), one as a triterpene (ursolic acid), and one as a megastigman glycoside (3-oxo--ionol -glucose). The antioxidant, cytotoxic, anti-inflammatory, and wound-healing effects of the extracts, fractions, and pure compounds were investigated. Antioxidant effects were determined using DPPH, SO, and ABTS radical scavenging methods, while cytotoxic effects were investigated using the MTT method. The anti-inflammatory effect was measured in LPS-stimulated RAW 264.7 macrophages by quantification of NO, IL-6, TNF-, and IL-1. The wound healing effect was determined using a scratch test on L929 cells. The study results showed that the scavenging effect of P. major subsp. intermedia extract (DPPH IC50: 139.84; SO IC50: 108.34; ABTS TEAC: 130.95) against all three radicals was higher than that of P. scabra extract (DPPH IC50: 160.62; SO IC50: 316.53; ABTS TEAC: 283.40). In LPS-stimulated RAW 264.7 cells, the P. scabra Fr. fraction was found to be more prevalent among the fractions. In the wound healing experiment, P. major subsp. intermedia Fr. A (10 µg/mL: 83.31% inhibition) showed the highest NO inhibition among the fractions, while among the pure compounds, acteozide (100 µM: 61.89% inhibition) showed the highest inhibition. In the wound healing experiment, P. major subsp. intermedia Fr. A (10 µg/mL: 91.87% ± 5.28%) showed the highest effect among the fractions, while aucubin (6.25 µM: 69.33% ± 0.97%) showed the highest effect among the pure compounds. The findings contribute to the literature with detailed phytochemical and biological effect research of P. major subsp. intermedia and P. scabra plants.
Tarım Çalışanlarında Pestisit Maruz Kalımı ve Dikkat Düzeyi
(Sağlık Bilimleri Enstitüsü, 2026-03-12) NEZAKET ÖZPOLAT ÇAKAR; Halk Sağlığı
Özpolat Çakar N., Pesticide Exposure and Attention Level in Agricultural Workers, Hacettepe University, Graduate School of Health Sciences, Occupational Health Program PhD Thesis, Ankara, 2026. This thesis is a cross-sectional study conducted to examine the relationship between the risk of pesticide exposure and attention deficit level in agricultural workers, and the role of mental health status in this relationship. The study included 400 out of 1050 agricultural workers registered with the Erdemli Chamber of Agriculture in Mersin province. The sociodemographic characteristics, habits, work life characteristics, pesticide exposure, mental health status, and attention levels of the workers were evaluated. The risk of pesticide exposure was calculated based on a total risk score created by retrospectively questioning factors such as exposure duration, frequency and amount, toxicity of the pesticide used, age of exposure, receiving pesticide application training, and use of personal protective equipment. When attention deficit levels were examined, it was determined that 18 participants (4.5%) had levels consistent with an ADHD diagnosis and required clinical evaluation. In the multiple linear regression analysis conducted to determine the variables associated with attention deficit level; it was observed that employees with higher scores in alcohol use habit (p=0.042; 95% CI 0.03/1.41), daytime sleepiness (p=0.002; 95% CI 0.06/0.24), mental health status (p<0.001, 95% CI 0.41/0.57), and pesticide risk status (p=0.006 95% CI 0.03/0.17) had higher attention deficit levels. The inattention subscale was associated with alcohol use, presence of chronic disease, daytime sleepiness, and mental health status; the hyperactivity/impulsivity subscale was associated with alcohol use, mental health status, and pesticide risk status. The mediation analysis results showed that mental health status was not a mediating mechanism explaining the relationship between pesticide risk status and attention deficit level. In conclusion, it was assessed that attention deficit levels in agricultural workers are related to multiple factors, and that measures to strengthen mental health should be considered together with practices aimed at reducing pesticide exposure.
Cinsel Suçlarda Tıbbi Kastrasyon Uygulanmasının Tıp Etiği Açısından Değerlendirilmesi ve Bu Konuda Ürologların Bilgi ve Tutumları ile İlgili Bir Araştırma
(Sağlık Bilimleri Enstitüsü, 2026-03-10) Dadalı, Mümtaz; Tıp Tarihi ve Etik
This study offers a comprehensive ethical analysis of medical (chemical) castration for sexual offenders, focusing on the knowledge levels and ethical attitudes of urology specialists in Türkiye. Although chemical castration is presented as a method to prevent repeat offenses by suppressing sexual urges through hormonal treatment, it represents a serious intrusion on bodily integrity and autonomy, raising significant debates around human rights and medical ethics. The study examines the historical development, global legal frameworks, and comparative practices of chemical castration, evaluating regulations in United States of America states such as California, Florida, Iowa, Louisiana, Texas, Massachusetts, Alabama, and Arizona through the lenses of voluntariness, informed consent, proportionality, and human rights compatibility. Examples from European countries including Germany, Poland, Czechia, and Russia are also discussed. International ethical guidelines like World Medical Association Declarations and the Convention on Human Rights and Biomedicine (Oviedo Convention) are used to explore physicians’ professional responsibilities and ethical obligations. In this thesis, Survey results from urology specialists in Türkiye reveal heterogeneous knowledge levels and limited ethical awareness. While some participants view chemical castration as a potential tool for social protection, others highlight risks of human rights violations, lack of genuine voluntariness and informed consent, and threats to trust in the physician patient relationship. Many also note that making such treatment legally mandatory could conflict with core ethical principles of medical practice. The study argues that chemical castration should not be seen merely as a punitive measure but as a medical ethical intervention requiring careful evaluation based on voluntariness, informed consent, and respect for human rights. A feminist bioethics perspective further critiques how it medicalizes individual culpability while obscuring the structural roots of gender inequality and violence, advocating for more holistic, preventive, and socially grounded approaches. Ultimately, it emphasizes the need to redefine the role of healthcare professionals in this area with a strong sense of ethical responsibility and a human rights based framework.