Hacettepe Üniversitesi Açık Erişim Sistemi (HÜAES)
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OKSİJEN-18 TEMELLİ GC-MS İLE FARKLI KANSER HÜCRELERİNİN METABOLİK DİNAMİKLERİNİN KARŞILAŞTIRILMASI
(2026) Ceylin Ökten; Analitik Kimya
Cancer cells reprogram their energy metabolism to sustain rapid proliferation. Therefore, metabolomic and fluxomic approaches are important tools for investigating cancer metabolism. However, the reliability of the obtained data directly depends on sample preparation and metabolite quenching procedures. In this study, methanol, perchloric acid, and methanol–perchloric acid quenching methods were compared in A549 cells, and the selected method was subsequently applied to H218O-based fluxomic analyses in A549, Hep3B, and LoVo cell lines. Relative abundances of metabolites associated with amino acid metabolism, glycolysis, and the tricarboxylic acid (TCA) cycle were determined by GC-MS. Methanol generally provided higher and more balanced relative abundances across the investigated metabolite groups and was therefore selected for H218O labeling experiments. A549, Hep3B, and LoVo cells were incubated in medium containing 30% H218O for 5, 15, and 30 min, and relative 18O labeling profiles of selected metabolites were evaluated. TCA cycle metabolites showed higher H218O incorporation in Hep3B and LoVo cells than in A549 cells, whereas metabolites associated with glycolysis and phosphate metabolism generally exhibited higher relative labeling in A549 cells. Cell-line-specific differences were also observed in amino acid metabolism. In conclusion, the methanol-based quenching method provided a suitable sample preparation approach for GC-MS-based metabolomic and H218O-based fluxomic analyses, and the findings demonstrated distinct metabolic dynamics among the three cancer cell lines.
Role of interventional radiology in the management of iatrogenic urinary tract injury: the factors affecting the outcome
(2024-07-08) Düzgün, Selin Ardalı; Ünal, Emre; Çiftçi, Türkmen Turan; Öztürk, Ebru; Akhan, Okan; Akıncı, Devrim; Radyoloji
PURPOSE
To evaluate the efficacy of interventional radiological (IR) procedures in iatrogenic urinary tract injury and investigate the factors affecting the outcome.
METHODS
Fifty-eight patients (21 male) with a mean age of 50.3 ± 15.8 years referred for iatrogenic urinary
tract injury were enrolled in this study. Technical success was defined as (i) successful placement
of a nephrostomy catheter within the renal pelvis and/or (ii) successful antegrade ureteral stent
placement (double J stent) between the renal pelvis and bladder lumen. Complete resolution was
defined as maintained ureteral patency without an external drain and ureteral stent. The factors
that may affect complete resolution [ureteral avulsion, ureterovaginal fistula (UVF), history of malignancy/radiotherapy, and time to IR management] were also investigated. The receiver operating
characteristic analysis was performed to estimate the cut-off time point for the IR management
timing affecting complete resolution.
RESULTS
The technical success rate for nephrostomy and ureteral stent placement was 100% (n = 58/58)
and 78% (n = 28/36), respectively. In 14 patients, non-dilated pelvicalyceal systems were evident.
In 18 patients, no further intervention after percutaneous nephrostomy was performed due to (i)
poor performance status (n = 6) and (ii) reconstruction surgery upon clinicians’ and/or patients’
request (n = 12). Reconstruction surgery was required in 11 of the remaining 40 patients due to
failure of percutaneous treatment (n = 11/40, 27.5%). In six of the patients, ureteral stents could not
be removed due to the development of benign ureteral strictures (n = 6/40, 15%). Our complete
resolution rate was 57.5% (n = 23/40). Age, gender, type of surgery (endoscopic or open), side and
location of the injury did not statistically affect the complete resolution rate. The presence of ureteral avulsion, history of malignancy and radiotherapy individually or in combination significantly
affected the complete resolution rate negatively. The presence of UVF also had a negative effect on
the complete resolution rate; however, it did not reach statistical significance. Delayed intervention
was also a significant factor related to lower complete resolution. The optimal cut-off point of the
time interval for favorable clinical outcome was found to be 0–19th day following the surgery.
CONCLUSION
IR procedures are safe and effective in the management of iatrogenic urinary tract injuries. Antegrade ureteral stenting should be performed as soon as possible to establish ureteral integrity
without the development of stricture
Percutaneous nephrostomy in infants: a 20-year single-center experience
(Tıp Fakültesi, 2024-09-09) Taydaş, Onur; Ünal, Emre; Akıncı, Devrim; Şeker, Mehmet; Topçuoğlu, Osman Melih; Akhan, Okan; Çiftçi, Türkmen Turan; Radyoloji
Purpose: To investigate the safety and efficacy of the imaging-guided percutaneous nephrostomy (PCN) procedure in infants.
Methods: A total of 75 (50 boys; 66.7%) patients with a mean age of 121 days (range, 1-351 days) who underwent PCN over a period of 20 years were included in this retrospective study. For each patient, PCN indications, catheter size, the mean duration of catheterization, complications, and the procedure performed following nephrostomy were recorded. Technical success was determined based on the successful placement of the nephrostomy catheter within the pelvicalyceal system. Clinical success was defined as the complete resolution of hydronephrosis and improvement in renal function tests during follow-up. In patients with urinary leakage, technical and clinical success was determined based on the resolution of leakage.
Results: The technical success rate was 100%, and no procedure-related mortality was observed. In 11 patients (14.7%), bilateral PCN was performed. The most frequent indication of PCN was ureteropelvic junction obstruction (n = 41, 54.7%). Procedure-related major complications were encountered in two patients (methemoglobinemia and respiratory arrest caused by the local anesthetic agent in one patient and the development of urinoma caused by urinary leakage from the puncture site in the other). Mild urinary leakage was the only minor complication that occurred and only in one patient. Catheter-related complications were managed through replacement or revision surgery in 16 patients (21.3%).
Conclusion: Imaging-guided PCN is a feasible and effective procedure with high technical success and low major complication rates, and it is useful for protecting kidney function in infants.
Yoğun Bakım Hemşirelerinin Deliryuma İlişkin Bilgi Düzeyleri ve Öz-Yeterlikleri Arasındaki İlişkinin İncelenmesi
(Sağlık Bilimleri Enstitüsü, 2026-08-13) KAYNARCİ ZİŞAN; İç Hastalıkları Hemşireliği
. This cross-sectional, correlational study examined the relationship between intensive care nurses’ knowledge of delirium and their self-efficacy in delirium care. The study was conducted between March 2025 and January 2026 among 232 nurses working in adult tertiary intensive care units at Ankara Etlik City Hospital. Data were collected using the Nurse Information Form, the Delirium Knowledge Test for Intensive Care Nurses (DBT-YBH), and the Delirium Care Self-Efficacy Scale for Intensive Care Nurses (DBÖYÖ-YBH). G*Power 3.1.9.7 was used to assess the adequacy of the sample size for multiple regression analyses, while R version 4.4.1 and IBM SPSS Statistics version 23 were used for statistical analyses. Descriptive statistics, the Mann–Whitney U test, Spearman’s rank correlation analysis, and robust regression analysis were performed, with statistical significance set at p<0.05. The median age of the nurses was 26 years, and the median durations of professional and intensive care experience were both 3 years. Of the participants, 73.3% were female and 91.0% held a bachelor’s degree. Although 62.9% of the nurses had previously received information about delirium, an equal proportion reported being dissatisfied with their current level of delirium knowledge. The median total DBT-YBH score was 19.00 (Q1–Q3: 15–21), while the median total DBÖYÖ-YBH score was 50.00 (Q1–Q3: 41–56). Intensive care experience was positively and weakly correlated with the total DBÖYÖ-YBH score (rₛ=0.167; p=0.011). Nurses who had received information about delirium and those who were satisfied with their current level of delirium knowledge had significantly higher DBÖYÖ-YBH scores (p=0.009 and p=0.004, respectively). Similarly, nurses who held an intensive care nursing certificate, had received information about delirium, and were satisfied with their current level of knowledge had significantly higher DBT-YBH scores. A significant positive correlation was found between DBT-YBH and DBÖYÖ-YBH scores (rₛ=0.360; p<0.001). Two robust regression models were established to identify factors associated with delirium knowledge and self-efficacy in delirium care. The first model explained 19.5% of the variance in DBT-YBH scores, whereas the second explained 20.3% of the variance in DBÖYÖ-YBH scores. In the second model, the DBT-YBH score significantly and positively predicted the DBÖYÖ-YBH score (β=0.330; p<0.001). Overall, the findings suggest that greater knowledge of delirium is positively associated with higher self-efficacy in delirium care among intensive care nurses. Accordingly, regular, practice-oriented delirium education programs should be incorporated into continuing professional development, and the routine clinical use of standardized delirium assessment tools should be encouraged.
ALOPESİ TEDAVİSİNDE KULLANILMAK ÜZERE ÜÇ BOYUTLU (3B) YAZICI İLE BARİSİTİNİB YÜKLÜ NANOSİSTEM İÇEREN MİKROİĞNELERİN TASARIMI VE İN VİTRO-EX VİVO DEĞERLENDİRİLMESİ
(Sağlık Bilimleri Enstitüsü, 2026-08-12) Betül Gür; Farmasötik Teknoloji
Gür, B. Design and In Vitro-Ex Vivo Evaluation of microneedles containing Baricitinib Loaded Nanosystems Using 3D Printer for the Treatment of Alopecia, Hacettepe University Graduate School of Health Sciences Pharmaceutical Technology Doctor of Philosophy Thesis, Ankara, 2026. Alopecia areata (AA) is a chronic and relapsing autoimmune disorder targeting the hair follicle. To minimize the adverse effects associated with oral baricitinib (BAR) administration and enhance follicular targeting, BAR-loaded polycaprolactone (PCL) and poly(lactic-co-glycolic acid) (PLGA) nanoparticles and nanostructured lipid carriers (NLCs) were developed and incorporated into dissolving microneedles. The PCL, PLGA, and NLC systems optimized using a Box–Behnken experimental design had particle sizes of 173.1, 148.8, and 54.8 nm, respectively, with encapsulation efficiencies of 69.42%, 81.95%, and 90.26%. Nanocarrier-loaded PVP K30/PVA-based microneedles maintained structural integrity under 32 N, achieved 100% penetration through the first three layers of the Parafilm® M model, and completely dissolved in porcine skin within 30 min. In ex vivo studies using porcine ear skin, the highest follicular BAR deposition was observed with PLGA nanoparticles, whereas the highest 24-h cumulative permeation was obtained with NLCs. Microneedle application reduced BAR detection time in the receptor medium from 4 h to 30 min and increased follicular deposition by 1.69-, 1.93-, and 1.94-fold for the PCL, PLGA, and NLC systems, respectively. Among the microneedle formulations, follicular deposition was highest with PLGA NP-loaded microneedles (19.48%), followed by PCL NP-loaded microneedles (15.13%) and NLC-loaded microneedles (14.30%). Confocal microscopy images supported nanocarrier delivery to the epidermal and superficial dermal regions via the microneedle systems. In conclusion, the developed system was considered promising for local and follicular BAR targeting in the treatment of AA.