Sinovial Sıvı ve Sinovial Membran Kaynaklı Mezenkimal Kök Hücrelerin Osteojenik, Kondrojenik ve Tendonojenik Farklılaşma Özellikleri

Abstract

The aim of this study is to isolate mesenchymal stem cells from synovial fluid and to investigate the amount, viability and chondrogenic differentiation potential of these cells in vitro. In the first stage of the thesis, synovial fluid aspiration was performed from the knee and elbow joints of anesthetized young and adult sheep with a 3 G syringe with a 23 G needle under sterile conditions. In the next stage, isolation of mesenchymal stem cells from synovial fluid was performed. No MTT test was required for cell viability because the cells were viable and healthy cells with long fibroblastoid appearance, low granularity, adhesion and proliferation. Then, the multipotency properties of synovium (SMKH) derived mesenchymal stem cells under two conditions, osteogenic and adipogenic growth and differentiation medium, iv were investigated. Alizarin Red was used for osteogenic differentiation and Oil Red O was used for adipogenic differentiation. As a result, MSCs isolated from synovial fluid obtained from knee and elbow joints of sheep were morphologically prolonged fibroblast-like, viable cells that adhered to the culture vessel and proliferated. SMCCs showed osteogenic differentiation in 30-40% and adipogenic differentiation in 30-40%. Calcification and calcium depots were detected in the extracellular matrix of cells of SMKHs differentiated to osteoblast precursors at the end of the 21st day. Osteogenic differentiation was defined by calcium phosphate accumulation in Alizarin Red stained cells at the end of the 21st day. Adipocytes show fat cell phenotype. In other words, a large and round droplet of oil is formed in which triglycerides are collected.