Aktif ve Remisyon Dönemi Ülseratif Kolit Hastalarında Metabolomik Analizler

Abstract

Ulcerative Colitis (UC) is a chronic inflammatory disease of the gastrointestinal system and is characterized by biochemical and metabolic changes. In this study, it was aimed to contribute to the understanding of the biochemical mechanisms of the disease by using metabolomic analysis methods on blood samples taken from ulcerative colitis patients. For this purpose, blood taken from active ulcerative colitis patients, in remission period ulcerative colitis patients and healthy people without this disease were analyzed using Liquid Chromatography/Quadrupole-Time of Flight Mass Spectrometry (LC/Q-TOF MS) method using a C18 column and gradient elution program. The obtained raw data were analyzed with MZmine software. Metabolite profiles were evaluated by biological pathway analysis on the MetaboAnalyst platform and the relationship of the metabolites found with ulcerative colitis was revealed. As a result of the data evaluation; when FC>1.5 and p<0.05 statistically reliable peaks were detected, 81 metabolites were obtained between the active and control groups, 88 metabolites between the remission and control groups, and 32 metabolites between the active and remission groups. As a result of the literature review, a total of 60 of these metabolites were found to be associated with ulcerative colitis disease. The findings not only provide a new perspective for understanding the metabolic dynamics of ulcerative colitis but also hold promise for identifying potential treatment targets and biomarkers that can be used in disease monitoring.