Doksorubisin Uygulanan Üçlü Negatif Meme Kanserinde Taurinin Tümör Büyüklüğü ve Metastaz Üzerine Etkisi

Abstract

Gedikli, E., The Effect of Taurine on Tumor Size and Metastasis in Triple Negative Breast Cancer Treated With Doxorubicin, Hacettepe University Graduate School of Health Sciences Physiology Program PhD Thesis, Ankara, 2023. Triple Negative Breast Cancer (TNBC) is characterized by a deficiency of estrogen, progesterone receptor and human epidermal growth factor receptor-2. It has a high metastatic potential and poor prognosis and is therefore more difficult to treat than other types of breast cancer. Chemotherapy is applied as the main treatment method. Doxorubicin (Dox) is one of the chemotherapy agents widely used in breast cancer treatment. However, it causes toxic effects in various tissues. Therefore, it is of great importance to use agents that can minimize toxic effects and increase the response to treatment. Taurine (Tau) is an intracellular free β amino acid with anti-oxidant and anti-cancer functions found in many mammalian tissues. It has been shown to cause apoptosis in cancer cells and inhibit tumor growth. In our study, we aimed to investigate the effect of taurine on the apoptotic and anti-metastatic role of doxorubicin in TNBC. In this study, 34 BALB/c female mice aged 6-8 weeks were used. TNBC cells (4T1) were injected subcutaneously into the left mammary gland to form solid tumors. For 21 days, Taurine (100 mg/kg i.p.) was administered daily, while Dox (4 mg/kg i.p.) was administered every 5 days and the animals were sacrificed on the 22 nd day. Animal were weighted every day, and tumor diameter and volume were measured every 3 days. At the end of the experiment, lung and tumor tissues were weighed. Apoptotic and metastatic protein levels in tumor tissues were determined by ELISA, and histopathological examination was performed. Although there was no change in protein levels, it was observed that Dox decreased tumor cell dissemination, microvascularization and nodule formation in lung tissue, and induced apoptosis in tumor tissues. In conclusion, although the effect of taurine on the anti-cancer role of doxorubicin is limited, taurine may be useful in reducing the toxic effects of Dox on normal tissues. Key words: Taurine, Doxorubicin, Triple Negative Breast Cancer, Apoptosis, Metastasis This study was supported by Hacettepe University Scientific Research Projects (BAP) Coordination Unit (Project no: THD-2021-19181).