Vankomisine Bağlı Kırmızı Boyun Sendromu Geliştiren Hastalarda Mrgprx2 Reseptör Mutasyonlarının Değerlendirilmesi

Abstract

Vancomycin is a tricyclic glycopeptide antibiotic effective against Gram-positive bacteria, isolated from the bacterial species Amycolatopsis orientalis. Vancomycin use can lead to two types of hypersensitivity reactions: Red Man Syndrome (RMS) and anaphylaxis. Red Man Syndrome is caused by degranulation of mast cells and basophils. Mast cells possess a wide range of surface receptors, including the Mas-related G-protein-coupled receptor element X2 (MRGPRX2), which can trigger mast cell degranulation via IgE-independent pathways as well. Studies have shown that MRGPRX2 is activated by vancomycin and some other drugs, directly triggering mast cell degranulation and anaphylactic reactions. In the thesis study, single nucleotide polymorphisms (SNPs) in the gene segment encoding MRGPRX2 were investigated to assess the role of genetic susceptibility factors in patients exhibiting RMS due to vancomycin use. Single nucleotide polymorphisms, SNPs, might be linked to MRGPRX2 variants that alter its structure and make individuals prone to hyperactivation. A total of 60 samples were used, including 38 patients and 22 controls in the thesis study. Polymorphisms were detected in 11 out of 38 patients, while no polymorphisms were found in the control group. Considering the potential relationship between MRGPRX2 and pseudoallergies as well as inflammatory disorders, it is believed that future studies could shed more light on the functionality of MRGPRX2. This could lead to novel approaches for the treatment of diseases and the management of drug sensitivities.