Dosetaksel İçeren Nanopartiküllerin Formülasyonu ve in Vitro Değerlendirilmesi

Abstract

Nanotechnology is the science of controlling materials in the atomic and molecular level. Today, along with its various applications in different fields, nanotechnology stands out to be quite promising in the cancer treatment since there have been many studies done in the field of nanomedicine. Defined as one of the top causes of death, cancer is a complex and important disease which starts with uncontrolled cell division. Currently applied cancer treatments are limited to surgery, radiation therapy, and chemotherapy. These three methods may result in damaging the normal tissues or incomplete eradication. Due to the ability of a controlled release, pharmaceutical encapsulation into the nanoparticles reduces drug toxicity and can be targeted into the intended area; thus, it provides a solution to the problems encountered. Docetaxel is an anticancer agent that belongs to the taxoid family. In this study, nanoparticles have been developed that provide controlled release with reduced toxic effects of docetaxel. With this purpose in mind, poly(lactic-co-glycolic-acid) (PLGA) as a polymer, and polyvinyl alcohol (PVA) as a surfactant were used. Resomers of PLGA were used with different concentrations and were dissolved in different solvents. After in vitro characterization studies (particle size, zeta potential, encapsulation efficiency, release studies) effects of these variables on nanoparticle properties were examined. Selected optimum formulation through the prepared docetaxel loaded nanoparticles was used for cell culture studies in order to evaluate the cytotoxicity on MCF-7 cells. Prepared docetaxel loaded nanoparticles size were dispersed homogenously. In the prepared formulations of docetaxel, high encapsulation efficiencies were observed and in the release profiles initial burst effect was followed by controlled release. Cytotoxicity studies showed that docetaxel loaded nanoparticle formulation has caused more cytotoxic effect in comparison with plasebo nanoparticles. These studies have shown that the docetaxel loaded nanoparticles could be a new approach to the cancer therapy.