ZENGİNLEŞTİRİLMİŞ FLORESAN GÖRÜNTÜLEME İÇİN FLORESAN BOYALAR İLE ETİKETLENMİŞ İNORGANİK NANOPARTİKÜLLER

Abstract

Fluorescence imaging, instrumentation and probe development are a versatile tool for biological and preclinical studies, with continuous improvement in performance. Sensitive perception and visualization of deep targets in vivo is particularly difficult due to diffusion and absorption of light by tissues and autofluorescence emission from real chromophores. Fluorescent inorganic nanoparticles offer interesting optical properties that can be significantly different from organic dyes. The silica nanoparticles used as core were synthesised by the stöber method. Fluorescent labeling was done before inorganic coating was applied, for which Rhodamine was used. Silica nanoparticles were dispersed in water or ethanol and a certain amount of fluorescent dye was added and mixed for 24 hours. SiO2 / NPler is coated with inorganic layers by sol-gel method. Coating with titanium dioxide was applied on silica nanoparticles by sol-gel technique. Titanium tetra isopropoxide solution (precursor) prepared in ethanol was used. The SiO2@TİO2/ NPs produced is stored in the dark. Characterization and various images were obtained after synthesis of inorganic multilayer nanoparticles. The size distribution of the nanoparticles was investigated using the ZetaSizer instrument. TEM photographs of nanoparticles were taken before and after coating. Labeling of the dyes with nanoparticles was examined by means of a fluorescent microscope. Interactions of these nanoparticles with both bacterial and human cell lines have been investigated in cell culture media.