YENİ TİP KRANİYOFASİYAL PEEK MIKNATIS İMPLANT DAYANAKLARININ İNSAN EPİTEL HÜCRESİ ÜZERİNDEKİ SİTOTOKSİK ETKİLERİNİN İN-VİTRO İNCELENMESİ

Abstract

ÖZŞEN MERT, In vitro examination of the effects of new types of implant abutments produced from the combination of PEEK and magnet on human epithelial cells. Hacettepe University Faculty of Dentistry, Thesis in Prosthodontics, Ankara, 2023. In addition to many advantages of magnetic abutments, they have a significant disadvantage such as corrosion. To prevent this, it has started to be used by encapsulating it with titanium. The aim of this in vitro study is to examine the effect of PEEK-magnet, developed as an alternative to titan-magnets, on skin epithelial cells. For this purpose, the cytotoxicity of the new type of magnetic abutment produced with biomedical PEEK and the currently used magnetic abutment made of titanium (Steco x-line) was examined before and after the corrosion test. In the study, 5 groups were determined and 6 samples were prepared from each group. 1st group HaCat cells with Steco x-line titanium-magnet abutments, 2nd group HaCat cells with titanium-magnet abutments placed after corrosion, 3rd group HaCat cells with PEEK-magnet abutments. 4th group HaCat cells where PEEK-magnet was placed after corrosion. The 5th and control group are HaCat cells without any material inside. To see the cytotoxic effect of the samples, they were kept in skin epithelial cell culture (HaCat cell line) for 48 hours. They were then kept in the salt spray corrosion test for 96 hours to prevent corrosion. Before corrosion, an average cell viability of 71.6% in titanium-magnet and 96.9% in PEEK-magnet was found. After corrosion, an average cell viability of 44% in Titan-magnet and 86.8% in PEEK-magnet was found. In comparing the pre- and post-corrosion values of PEEK-magnet and titan-magnet, ttest for dependent groups was examined and one-way ANOVA test was used to determine whether there was a difference between pre-corrosion and post-corrosion between Titan magnet, PEEK-magnet and control groups. There was no significant difference between HaCat cells on which Peek-magnet abutments were placed and the control group before and after corrosion (p>0,05). A statistically significant difference was found between the HaCat cells on which Titan-magnet was placed, both before and after corrosion, and the HaCat cells on which PEEK-magnet was placed and the control group (p<0.05). As a result, it has been shown that Peek-magnets are less itotoxic than Titan-magnets and can be used as an alternative. Keywords: craniofacial abutments, titanium, PEEK, corrosion, cytotoxicity