Protein A Baskılanmış Süpermakrogözenekli Poli (Hidroksietil-Metakrilat) Kriyojeller

Abstract

Protein A can be combined with Fc receptors of certain immunoglobulin (IgG1, IgG2, IgG4), and thus can be antifagositer and anticomplementer activity. In addition, this protein was used as a nonspecific carrier with S. aureus coaglutination constitutes the basis of tests. Protein A is the most important ligand of IgG purification, due to the connection of specific IgG. Therefore Protein A is great importance commercially.Molecular imprinting is a technology to create recognition sites in a macromolecular matrix using a template molecule. Molecularly imprinted polymers (MIP) are easy to prepare, stable, inexpensive and capable of molecular recognition. MIPs can be considered as affinity separation media.Cryogels are gel matrices that are formed in moderately frozen solutions with radicalic polymerization. The unique structure of cryogels, in combination with their osmotic, chemical and mechanical stability, makes them attractive matrices forchromatography of biological nanoparticles