Cytotoxic Effect Of Endodontic Irrigants In Vitro
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Date
2014Author
Bajrami, Donika
Hoxha, Veton
Gorduysus, Omer
Muftuoglu, Sevda
Zeybek, Naciye Dilara
Küçükkaya, Selen
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Background Cytotoxicity of root canal irrigants is important due to their close contact with host tissues. The aim of this study was to assess the cytotoxic effect of NaOCl 3%, Chx 2%, and MTAD on rat periodontal ligament fibroblasts, at 0.1 and 100 μl/mL, using WST-1 colorimetric method. Material/Method Rat ligamental fibroblasts were exposed to the irrigants and their viability was assessed after 1, 24, 48, and 72 h. The measurements were determined using WST-1 assay, using a micro ELISA reader. Results At 100 ml/L all 3 irrigants were strongly cytotoxic, although CHX was less so than NaOCl and MTAD. At the 0.1 ml/L concentration, NaOCl and MTAD were only moderately cytotoxic, whereas Chx was highly deleterious to cell viability at all time points. There was a significant influence of the dilution rate of the substance, because the odds ratio for cell viability being over 50% was increased 51 times between the 100 ml/L and 0.1 ml/L dilutions. Conclusions It seems that irrigating solutions should be used at lower concentrations to enhance cell viability.
URI
https://doi.org/10.12659/MSMBR.890247https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3958565/
http://hdl.handle.net/11655/19045