Sıçanlarda Maternal Diyetle Fruktoz Alımının Fetüse Etkilerinin Bazı Lipit Parametreleri ve Lipogenez Açısından İncelenmesi

Abstract

The aim of this study was to examine the effects of high fructose intake during preconception, throughout pregnancy and lactation on some lipid parameters and insulin level in the plasma, triglyceride accumulation in the liver and total body fat accumulation of both the mother rats and pups. Therefore, this study was carried out on Sprague Dawley strain, virgin female rats (n=10). After a two- week wash out period rats were divided into two groups. The fructose with a concentration of 0.2 g/mL (20% w/v) in water was administered to the first group and the same amount of maltodextrin was administered to the other group. After a 12-week dietary manipulation period the rats were mated for pregnancy. The dietary manipulation continued in the same way during pregnancy and lactation periods. At the end of lactation period, blood and issues were isolated from the rats and animals were immediately sacrificed. As a result, it was found that there were no differences on chow intake while water intake of maltodextrin group was higher (p=0.016). Body weights, plasma and liver trygliceride levels, plasma free fatty acids of both mothers and pups were found similar. Plasma insülin levels were significantly different in the mothers and pups (p=0.049 and p= 0.005, respectively). Total body fats of mothers in the fructose group were higher (p=0.029) but there was no difference in total body fats of pups between the two groups. Consequently, it was found that high fructose intake elevated plasma insülin level and total body fat and high fructose intake with maternal diet elevated plasma insülin levels. In conclusion, this study showed that to reduce the risk of chronic diseases such as type II diabetes mellitus and obesity in the later life of children, fructose intake in preconception, pregnancy and lactation periods might be restricted.