Perflorooktanoik Asitin Sitotoksisite Ve Oksidatif Stres Indükleyici Etkilerinin In Vivo Ve In Vitro Sistemlerde Araştırılması

Abstract

Perfluorooctanoic acid (PFOA) is a persistent environmental pollutant of toxicological importance. However, its mechanism of toxicity was not well-characterized. The aim of this study was to investigate the possible cytotoxic and oxidative stress inducing effects of PFOA in in vivo and in vitro model systems in the presence or absence of taurine and coenzyme Q10 (CoQ10). For this purpose, in the in vivo part of the study, animals were treated with two different concentrations (15 or 30 mg/kg/day) of PFOA by oral route for 10 days and lipid peroxidation, total glutathione levels, and activities of antioxidant enzymes (catalase, glutathione peroxidase and Cu-Zn-superoxide dismutase) were measured in liver, brain and kidney tissues. A significant and dose-dependent decrease in body weights and hepatomegaly in addition to reduction in kidney weights were observed in animals treated with PFOA. Evaluation of MDA levels and enzymatic and non-enzymatic antioxidant parameters showed that especially lower concentration of PFOA induced lipid peroxidation and a modification on the antioxidant systems. Pre-treatment of mice with taurine or CoQ10 before the administration of 30 mg/kg of PFOA provided a marked protection against PFOA-induced lipid peroxidation in liver tissue. In the in vitro part of the study, cytotoxic effects of PFOA was determined by MTT assay, and it was found that the effect of the substance on the viability of vero cells was time dependent. Cytotoxicity was observed ~≥ 200 μM concentrations of PFOA. Taurine or CoQ10 pretreatment was partially protective against PFOA-induced cytotoxicity. These results suggest the involvement of reactive oxygen species in the mechanism of toxicity of PFOA.