Perflorooktanoik Asitin Sitotoksisite Ve Oksidatif Stres Indükleyici Etkilerinin In Vivo Ve In Vitro Sistemlerde Araştırılması
Özet
Perfluorooctanoic acid (PFOA)
is a persistent environmental pollutant of toxicological importance. However,
its mechanism of toxicity was not well-characterized. The aim of this study was
to investigate the possible cytotoxic and oxidative stress inducing effects of
PFOA in in vivo and in vitro model systems in the presence or absence of
taurine and coenzyme Q10 (CoQ10). For this purpose, in the in vivo part of the
study, animals were treated with two different concentrations (15 or 30
mg/kg/day) of PFOA by oral route for 10 days and lipid peroxidation, total
glutathione levels, and activities of antioxidant enzymes (catalase, glutathione
peroxidase and Cu-Zn-superoxide dismutase) were measured in liver, brain
and kidney tissues. A significant and dose-dependent decrease in body
weights and hepatomegaly in addition to reduction in kidney weights were
observed in animals treated with PFOA. Evaluation of MDA levels and
enzymatic and non-enzymatic antioxidant parameters showed that especially
lower concentration of PFOA induced lipid peroxidation and a modification on
the antioxidant systems. Pre-treatment of mice with taurine or CoQ10 before
the administration of 30 mg/kg of PFOA provided a marked protection against
PFOA-induced lipid peroxidation in liver tissue. In the in vitro part of the study,
cytotoxic effects of PFOA was determined by MTT assay, and it was found that
the effect of the substance on the viability of vero cells was time dependent.
Cytotoxicity was observed ~≥ 200 μM concentrations of PFOA. Taurine or
CoQ10 pretreatment was partially protective against PFOA-induced
cytotoxicity. These results suggest the involvement of reactive oxygen species
in the mechanism of toxicity of PFOA.