Dişeti Oluğu Sıvısı ve Peri-implant Oluk Sıvısında Sklerostin, TWEAK, RANKL ve OPG Oranlarının Karşılaştırılması

Abstract

The combination of local and systemic factors plays a role in the pathogenesis of periodontal and peri-implant diseases. Host-derived enzymes, cytokines and other proinflammatory mediators play an integral role in this destruction. The aim of this study is to detect gingival crevicular fluid (GCF) and peri-implant crevicular (PICF) fluid levels of sclerostin, TWEAK, RANKL and OPG in periodontal and peri-implant tissues in disease and health conditions and also to assess the potential for use as biomarkers. The study population was consisted of 50 women and 41 men, in total of 91 individuals, with a mean age of 51.84 ± 14.05. Periodontitis (n=22), periodontal health (n=17), peri-implantitis (n=27) and 'peri-implant health' (n=25) groups were established according to clinical and radiographic examination results of 39 teeth and 52 implants restored with fixed prosthetic restorations. In all groups, measurements of periodontal and peri-implant parameters (probing depth, gingival recession, gingival bleeding time index, gingival index and plaque index) were performed and GCF and PICF samples were also collected. Sclerostin, TWEAK, RANKL and OPG levels in GCF and PICF were measured with ELISA tests. ‘Diseased’ groups revealed significantly higher TWEAK, RANKL and OPG levels compared to ‘healthy’ groups. Higher sclerostin levels have been detected in peri-implantitis groups when compared to peri-implant health group. In this study which evaluates four different bone metabolism products at the same time, sclerostin is thought to be a biomarker for peri-implant disease diagnosis due to the significantly higher levels in the peri- implantitis group than in the peri-implant health group. Moreover, TWEAK is found to be a better biomarker for both periodontal and peri-implant disease, due to the correlations with periodontal clinical parameters and the higher levels of TWEAK in disease conditions compared to the health in both dental implants and teeth.