Polimerik Nanopartiküllerin Partikül Büyüklüğüne Bağlı Olarak Hücre İçine Alım Kinetikleri ve Mekanizmaları

Abstract

In this study, it was aimed to investigate cellular uptake of PLGA and chitosan nanoparticles depending on particle size. For this aim, PLGA and chitosan nanoparticles were prepared by nanoprecipitation and ionic gelation method, respectively. PLGA nanoparticles’ average particle size was found stable in cell culture medium but chitosan nanoparticles were aggregated. Based on this results, effect of nanoparticles’ size on intracellular delivery was evaluated using PLGA nanoparticles on HEK293 cells. On the other hand, effect of chitosan nanoparticles’ aggregation on human brain endothelial cells (hCMEC/D3 cells) and chitosan nanoparticles’ interactions was evaluated using brain-targeted chitosan nanoparticles (Chitosan-PEG-Biotin-CD71). In conclusion, our findings showed that a small variation in the average particle size of PLGA nanoparticles prepared by nanoprecipitation can change the nanoparticles’ characteristics, influence their efficacy for cellular delivery and in vivo behaviors. Also, hCMEC/D3 cells used macropinocytic and receptor-mediated endocytic pathways to uptake Chitosan-PEG-Biotin-CD71 nanoparticles.