Kapiler Elektrokromatografi İçin Yeni Monolitik Sabit Fazların Sentezi ve Karakterizasyonu

Abstract

In the scope of this thesis, the synthesis of polymethacrylate based capillary monoliths was performed via block copolymerization by using a new hydrophilic monomer with reactive chloropropyl moiety, 3-chloro-2-hydroxypropyl methacrylate (HPMA-Cl). These columns were successfully tried in the electrochromatographic separation of alkylbenzenes, phenols, polyaromatic hydrocarbons, nucleosides, nucleotides and the highest theoretical plate counts was achieved as 205.000 plates m in these separations. The results indicated that a new reactive monolith in the form of polyacrylate was obtained and the synthesis of new electrochromatographic stationary phases could be achieved by using this reactive monolith. Hence, a new alternative to the commonly utilized reactive structures like poly(chloromethylstyrene-co-divinylbenzene) and poly(glycidyl methacrylate-co-ethylene dimethacrylate) was developed.