Östradiol Tanıma Bölgelerine Sahip Yüzey Plazmon Rezonans Sensörlerin Hazırlanması

Abstract

The aim of this study is the preparation of 17-β-estradiol (E2) imprinted surface plasmon resonance sensor for detecting E2. Fort this reason, SPR biosensors were modified with allyl mercaptane and the orientation of allyl groups was ensured. In the first step, the complex was prepared using E2 and N-Methacryloyl-(L)-leucine methyl ester (MALM) monomer and prepared complex with functional monomer, HEMA and crosslinker, EGDMA was polimerized on the golden surface of SPR under UV light. E2 imprinted SPR chip was characterized with FTIR-ATR, ellipsometry, atomic force microscopy (AFM) and contact angle measurement. A new chip was prepared without E2 in order to obtain E2 nonimprinted SPR sensor and the chip was characterized with ellipsometry, AFM and contact angle measurement. Adsorption studies were performed using carbonate buffer (pH 11) and desorption studies were performed with 20% ethylene glycol.