Nanomodifiyeli Kardiyopleji Solüsyonunun Miyokard Korunmasındaki Etkisinin Gözlenmesi

Abstract

In this thesis study, the effects of cardioplegia solutions modified with Poly(2-hydroxyethyl methacrylate) (PHEMA) and Poly(2-hydroxyethyl methacrylate) - N-methacryloyl-L-histidine methyl ester (PHEMAH) nanoparticles on myocardial protection was investigated in-vitro. To characterization the synthesized nanoparticles; zeta size analysis, scanning electron microscopy (SEM), Fourier Transform Infrared Spectroscopy (FT-IR), swelling experiments and surface area measurements were performed. Adsorption behaviors of PHEMAH nanoparticles were explained by investigating the interactions between nanoparticles and L-histidine. Effects of adsorption of L-histidine; different buffer solutions, initial concentration of L-histidine, adsorption time and temperature were investigated. The maximum adsorption capacity was reached at Tris buffer, at 1.5 mg/mL initial concentration of L-histidine and at 45 minute adsorption time. The maximum adsorption capacity was found as 251.2 mg/g. Effects of Histidine, Tryptophan, Ketoglutarate (HTK) solutions in different volumes containing different nanoparticle consantrations were compared with 3- [4,5-dimethyl-2-thiazolyl] -2,5-diphenyl tetrazolium bromide (MTT) test in-vitro. Success between groups was demonstrated for the role of myocardial protection in the HUVEC cell line. This study showed that cardioplegia solution which was modified with PHEMA and PHEMAH nanoparticles provided better cell viability percentage at a lower volume as compared to HTK and has a potential to support myocardial protection.