Deneysel Sıçan Beyin Tümörü Modelinde Polilaktik–Ko-Glikolik Asit (Plga) Nanopartikülüne Yüklü Curcuminin Etkisinin Izlenmesi

Abstract

Glioblastoma multiforme (GBM ) is one of the most malignant brain tumors. With radical surgery, radiotherapy and chemotherapy median survival is 12-14 months. Due to the infiltrative and invasive nature of glioblastoma may spread to the entire brain. The most important limiting factor in the treatment of glioblastoma is blood brain barrier (BBB ). This barrier prevents chemotherapeutic agents reache into the tumor cells and without the adverse side effects of chemotherapeutic drugs at therapeutic doses do not have access to the tumor. To overcome this, the lipid -coated nanoparticles is observed using as few systemic side effects and drug delivery to achieve higher drug concentration in the tumor tissue and the treatment efficiency is significantly increased. Plants in this study performed in turmeric " curcumin " has anti tumor active ingredient polylactic - co - glycocholic acid ( PLGA) was charged to the nanoparticles and the antitumour activity was tested in the rat RG2 glioma model. In this study, control, intravenous (IV) and intratumoral ( IT) empty nanoparticles and IV and IT groups curcumin loaded nanoparticles were formed. Tumor formation was confirmed by magnetic resonance imaging, during treatment and 5 days after administration was compared by measuring the dimensions of the MRI. In the control group, tumor size was increased significantly over time (33.9±21.3 mm3 - 123.7±41.1 mm3; p=0.036). The most pronounced tumor shrinkage was observed in the IT administered curcumin-loaded nanoparticle group (66.6±44.6 mm3 - 34.9±21.7 mm3; p=0.028). Although the reduction of tumor size in the IT curcumin-loaded nanoparticle group was not statistically significant (20.9±13.2 mm3 - 9.0±6.8 mm3; p=0.249), this change in tumor size was significant when compared to the control group (p=0.036). IV curcumin was also effective in limiting the tumor growth to some extent, but, tumor shrinkage was not achieved with this treatment (17.7±21.2 mm3 - 18.7±18.9 mm3; p=0.528). The experiments in the study showed that curcumin-loaded PLGA nanoparticles, especially when administered via intracerebral route, reduce tumor size as demonstrated by both MRI and histopathologic investigation.