Çinko Oksit ve Bakır Oksit Nanoparçacıklarının in Vitro Sistemler Üzerindeki Toksik Etkilerinin Araştırılması
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Date
2017-09Author
Paslı, Duygu
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Zinc oxide (ZnO) and
copper oxide (CuO) NPs are commonly used metal nanoparticles with a high toxicity
potential. However, information on toxicity mechanisms like other NPs, is limited. In
this study, the possible cytotoxic effects of ZnO and CuO NPs on cell lines, either alone
or in combination, on mouse fibroblast (L929) and human neuroblastoma (SH-SY5Y)
cell lines were assessed and the protective effects of diethylenetriaminepentaacetic
acid (DTPA), N-acetylcysteine and taurine have been examined. As a result of MTT
and neutral red (NR) experiments, a time- and dose-dependent cytotoxic effect was
observed with ZnO NPs, time-dependent cytotoxic effect with CuO NPs and
concentration-dependent cytotoxic effect with mixture in L929 cells. Reactive oxygen
species (ROS) and mitochondrial membrane potential (MMP) increased, apoptosis
and intracellular calcium levels were found to be lower than control levels. CuO and
mixture induced autophagy and in the presence of NAC and taurine, these levels were
decreased and no increase in IL-6 production was observed. In SH-SY5Y cells, MTT
and NK tests resulted in lower cytotoxicity than observed in L929 cell line. It was
determined that ROS levels and MMP were elevated with the mixture. Apoptosis was
induced, intracellular calcium levels was decreased with this NPs alone or in
combination; IL-8 levels were not detected and no increase in IL-6 levels were
observed.