ZENGİNLEŞTİRİLMİŞ FLORESAN GÖRÜNTÜLEME İÇİN FLORESAN BOYALAR İLE ETİKETLENMİŞ İNORGANİK NANOPARTİKÜLLER
Özet
Fluorescence imaging, instrumentation and probe development are a versatile tool
for biological and preclinical studies, with continuous improvement in performance.
Sensitive perception and visualization of deep targets in vivo is particularly difficult
due to diffusion and absorption of light by tissues and autofluorescence emission
from real chromophores. Fluorescent inorganic nanoparticles offer interesting optical
properties that can be significantly different from organic dyes. The silica
nanoparticles used as core were synthesised by the stöber method. Fluorescent
labeling was done before inorganic coating was applied, for which Rhodamine was
used. Silica nanoparticles were dispersed in water or ethanol and a certain amount of
fluorescent dye was added and mixed for 24 hours. SiO2 / NPler is coated with
inorganic layers by sol-gel method. Coating with titanium dioxide was applied on
silica nanoparticles by sol-gel technique. Titanium tetra isopropoxide solution
(precursor) prepared in ethanol was used. The SiO2@TİO2/ NPs produced is stored
in the dark. Characterization and various images were obtained after synthesis of
inorganic multilayer nanoparticles. The size distribution of the nanoparticles was
investigated using the ZetaSizer instrument. TEM photographs of nanoparticles were
taken before and after coating. Labeling of the dyes with nanoparticles was examined
by means of a fluorescent microscope. Interactions of these nanoparticles with both
bacterial and human cell lines have been investigated in cell culture media.
