Doku Genişletme Amaçlı Hidrojel Sentezi, Karakterizasyonu ve Şişme Kinetiği

Abstract

This thesis was prepared with the support of TÜBİTAK Project, named Yeni Bir Osmotik Yumuşak Doku Genişletici Geliştirilmesi: İn-vitro ve İn-vivo Çalışmalar with the code number 111S120. The main goal of this research is to develop a hydrogel system, which is eliminating the problems seen in current soft tissue expander, for used in reconstructive and plastic surgeries such as the closure of tissue defects. For this purpose, hydrogels with lower swelling rate and higher swelling capacity were produced from the polymers which are more biocompatible from current expander with the size as small as possible. Then, to reduce and control gel swelling, hydrogels with desired swelling kinetic covered with silicon shell and the pore on the shell was closed by PCL nanofiber to allow wound healing for approximately the first ten days.Hydrogels systems were prepared by a free radical chain polymerization and cross-linking. 2-hydroxyethyl methacrylate (HEMA), acrylic acid (AAc), acrylamide(AAm), methyl methacrylate (MMA) and a natural polymer carboxy methyl cellulose (CMC) were used as monomers and with these monomers, p(HEMA-koCMC), p(AAm-ko-AAc), p(AAm-ko-HEMA) and p(AAm-HEMA-MMA) hydrogels having different compositions were synthesized. N,N’-Methylenebis acrylamid (BIS) was used as a crosslinker and ammonium persulphate (APS) and N,N,N’,N’-Tetramethylenediamine (TEMED) was used as the redox initiator pair. Synthesized hydrogels had 3 mm diameter and 10 mm length.