Mezenkimal Kök Hücrelerin Osteojenik Farklılaşmasında Çeşitli Biyoreaktör Performanslarının Karşılaştırılması

Abstract

The aim of this study is to investigate the growth and osteogenic differentiation of mesenchymal stem cells in tissue engineering bioreactors supported with scaffolds. For this purpose, chitosan-hydroxyapatite (HA) superporous hydrogel composites (chitosan-HA SPHCs) and mineralized silk scaffolds were used and dynamic culture studies were carried out with spinner flask bioreactors and perfusion bioreactors in comparison to static culture.Chitosan-HA SPHCs were synthesized by combining microwave irradiation and gas blowing techniques. The porous, three-dimensional structures of hydrogels were visualized by scanning electron microscopy (SEM) and micro-computed tomography (µCT) analyses. After a 28-day culture realized in the spinner flask bioreactor at 50 rpm stirring rate, mitochondrial activities and alkaline phosphatase activities of rat adipose tissue derived mesenchymal stem cells (rAdMSCs) were found to be comparable under static and dynamic conditions.