Effects Of Propranolol And Paclitaxel On Angiogenesis In Breast Cancer Cell Lines

Abstract

Purpose: The aim of this study was to investigate the effect of propranolol (PR), which is used in infantile hemangiomas, and paclitaxel (PX), which is widely used as an chemotherapeutic agent, on cancer cells. Materials and Methods: That the cells counted with trypan blue the doubling time were determined. Also with MTT assay were analyzed the cytotoxic effect and IC50 value of drugs. In the breast cancer cell lines which are differents with regard to invasion (MDA-MB-231 and MCF-7) anti-VEGF, anti-eNOS, anti-iNOS and anti-ERK1/2 primer antibodies investigated by using immunohistochemical methods. To evaluation of immunoreactivity was used the H-scoring system. Results: With MTT test, IC50 values are applied to the cells dosage for MDA-MB-231 cells; PX: 5 nmol, PR: 50 mu m, and for MCF-7 cells PX: 3,7 nmol, PR: 50 mu m, were established. In immunohistochemical application, immunoreactivity of control group was increased with strong and/or stronger in the cancer cells, while those of in PX, PR and combine treatment was decreased either significant or very significant. Conclusion: With this study, application of anti chemotherapeutic therapy which is paclitaxel, in additon with anti angiogenic therapy in the treatment of breast cancer, vascular vasodilation, cell proliferation, migration, survival ultimately thought to be important in the prevention or reduce of angiogenesis.