Parenteral Lipid Emülsiyonlarının Deney Fare Sepsis Modelinde Mezenterik Hipoperfüzyon, Oksidatif Stres ve Organ Hasarına Etkisi

Abstract

Öz, G., Effects of Parenteral Lipid Emulsions on Mesenteric Hypoperfusion, Oxidative Stress and Organ Damage in Experimental Mouse Sepsis Model, Philosophy of Doctorate (PhD) Thesis in Medical Pharmacology, Ankara, 2020. In this study, the effect of different parenteral lipid emulsions on mesenteric hypoperfusion, inflammation-induced oxidative damage and organ damage in the septic shock model created with lipopolysaccharide in mice was investigated. When mesenteric blood flow was evaluated, the flow decreased significantly in animals treated with lipopolysaccharide (ml/min, control: 3.02 ± 0.10, LPS: 1.44 ± 0.10). Olive oil and soybean oil decreased mesenteric blood flow in the control group. Reduced mesenteric blood flow and fish oil decreased with lipopolysaccharide but this difference was not observed when the data was interpreted according to body weight. Lipid emulsions did not prevent the increased liver and spleen wet weight after the application of lipopolysaccharide. Soybean oil statistically significantly increased the oxidative stress index in liver, lung and serum. Olive oil reduced the oxidative stress index in spleen, which increased with lipopolysaccharide, but increased in serum in the control group. In histopathological examination, lipid emulsions in the doses used show histopathologically harmful effects for healthy mouse liver and spleen. In the presence of lipopolysaccharide, fish oil does less damage to the liver than just lipopolysaccharide application. The main finding of these results is that fish oil emulsions containing high omega-3 fatty acids increase decreased mesenteric blood flow and prevent liver damage in the experimental sepsis model. Fish oil emulsions cause organ damage in healthy animals in sepsis-induced mice at doses with protective effects.