Rhodotorula Mucilaginosa Dan Lipaz Enzimi Üretimi ve Aktivitesine Etkili Parametrelerin Belirlenmesi

Abstract

Lipases (E.C.3.1.1.3; tri glycerol acyl hydrolases) are hydrolizer enzymes which catalyze free fat into glycerol and triglycerid. Industrial lipase commonly is provided by external cellular activity from strains of microorganisms. Microbial lipases are produced in liquid phase and its activity is influenced by concentrations of carbon nitrogen resources, pH, temperature and metal ions. In this study, Rhodotorula mucilaginosa as a lipase source, isolated from the village which is located near Eskisehir city. In the production of lipase, environmental conditions and media components are investigated as a important parameter. Vegetable oils as carbonivsource (olive oil, sunflower oil, soybean oil, corn oil and peanut oil) and carbohydrate source (gluconic, galactose, fructose, lactose, maltose and sucrose) and nitrogen sources (peptones, ammonium sulfate, urea, yeast extract, casein, ammonium oxalate, ammonium carbonate and proteose peptone) are used for lipase activity investigation