Nandrolonun Genotoksisitesinin Tek Hücre Jel Elektroforez Tekniği İle İn Vitro İncelenmesi
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Date
2019Author
Korkmaz, Emre
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Nandrolone decanoate is
among the most widely used anabolic androgenic steroids (AAS) that are often abused by
young people and athletes to improve muscle strength and performance. It is a subjected
to control in many countries and so non-medical use is generally illicit. It is known to
adversely affect health. Despite the risks of significant side effects, it is commonly abused.
It has high dose and long term intake. As a results of literature review, it seems that a few
studies have been conducted on the genotoxicity of nandrolone decanoate, which are
insufficient. The aim of this study was to evaluate the effects of nandrolone decanoate on
cell viability in V79 Chinese hamster fibroblast cells in a wide range of doses and DNA
damage in human lymphocytes at non-cytotoxic doses. The cytotoxicity was determined
using MTT assay. Genotoxicity of nandrolone decanoate in human lymphocytes as well
as the effect of H2O2-induced oxidative DNA damage was determined by alkaline single
cell gel electrophoresis method (Comet assay). Nandrolone decanoate significantly
decreased the cell viability in a dose dependent manner at the concentrations higher than
250 μM in V79 cells. IC50 dose was found to be 739 μM. Nandrolone decanoate alone
did not lead to DNA damage in the lymphocytes at all studied concentrations (0.5-100
μM). It seemed to significantly reduce oxidative DNA damage at the concentrations
between 10-100 μM in a dose-dependent manner, but not to change at the lower
concentrations (0.5 and 1 μM). The results of study show that nandrolone decanoate may
decrease oxidative stress-induced DNA damage at non-genotoxic doses. In conclusion,
the possible antigenotoxicity of nandrolone against the oxidative stress observed in our
study may contribute to the current scientific studies in revealing the mechanism of
toxicity; however, further research is needed.