Deneysel Meme Kanseri Modelinde Splenektominin İmmünolojik Etkisi

Abstract

Sevmiş M. Immunologic effects of splenectomy in experimental breast carcinoma model. Hacettepe University School of Medicine, General Surgery Residency Thesis, ANKARA 2015. Immune system plays an important role in the formation, distribution and destruction of cancer cells. Myeloid derived cell located into the tumor tissue may contribute to development of malignancy, invasion and vascularization. Spleen is the primary location of myeloid derived cells. The aim of this study is to investigate the effect of splenectomy on myeloid derived cells and cancer development in experimental breast carcinoma model. Totally 38 Balb-c 6 week-old mice were included in the study. Mice were separated into 5 groups: control, breast carcinoma, splenectomy, splenectomy before breast carcinoma and splenectomy after breast carcinoma. Tumor size, body weight and general condition of mice were checked twice a week. Experiments were terminated three weeks after tumor implantation. Myeloid derived cells in blood, liver and spleen samples were analyzed by flow cytometric immunophenotyping. Later the organs were histopathologically evaluated. A short-term weight loss was observed postoperatively. This weight loss was more prominent in cancer groups. Leucocytosis was observed in splenectomized mice. Primer tumor was decreased in splenectomized mice but liver and lung metastasis were increased. Splenectomy was observed in non-splenectomized tumorous mice. Immunologic analysis revealed high loads of myeloid derived cells and immature granulocytic cells in liver and spleen. Both immature granulocytic cells and immature monocytic cells were increased in blood and liver after splenectomy. Splenectomy affects the distribution of myeloid and tumor cells directly, even when breast carcinoma does not methastasize to the spleen directly. Splenectomy increases hepatic and pulmonary methastasis and also increases body weight, parallel to myeloid derived cell distribution