Bor İçeren Nano Hidroksiapatit Kompozitlerin Kemik Hücrelerindeki Moleküler Etki Mekanizmasının Araştırılması
Özet
The structure of the bone and the self-renewal capacity are damaged by metabolic diseases
and damages. The trace elements and hydroxyapatite crystals in the bone are important in
the development of biomaterials to support the renewal of the extracellular matrix. In this
study, it was assumed the boron loaded nanometer sized hydroxyapatite was supposed to
support the construction of extracellular matrix by controlled boron release in order to
prevent toxic effect. In this context, boron release kinetics from nanometer sized
hydroxyapatite were calculated by ICP-MS method and it was found that boron was
released in large proportion within 1 hour and the release was continued at constant low
dose. The effect of the boron containing nanometer sized hydroxyapatite composite on the
proliferation of SaOS-2 osteoblasts and human bone marrow derived mesenchymal stem
cells was evaluated by the WST-1 method and compared with the effects of
nanohydroxyapatite and boric acid, it has been shown that do not alter the proliferation rate
in osteoblasts and increase proliferation in mesenchymal stem cells at high doses. Boron
containing nanohydroxyapatite composites have been shown to increase osteogenic
differentiation of mesenchymal stem cells by increasing alkaline phosphatase activity, as
compared to nanohydroxyapatite composite and boric acid. The molecular mechanism of
effective dose of boron-containing hydroxyapatite has been assessed by transcriptomic
analysis and shown to affect genes involved in Wnt, TGF-β and response to stress
signaling pathways when compared to nano-hydroxyapatite composite and boric acid.
Eventually, a safe osteoconductive dose range of boron-containing nano hydroxyapatite
composites for local repair of bone injuries and the molecular effect profile in the effective dose was determined has been determined. Further studies require the validation of the
regenerative therapeutic effect window.
