Deneysel Meme Kanseri Modelinde Karaciğer Rezeksiyonunun İmmünolojik Etkileri

Abstract

Liver is the third common solid organ of breast cancer metastasis. If it is left untreated, the overall survival is only 4-8 months. Even though, liver resection for liver metastasis is shown to improve the survival, acceleration of hepatic and intrahepatic tumor proliferation during liver regeneration due to inflammatory cytokines is controversial. The aim of this study is to evaluate the effect of liver resection on different chemokine profiles in experimental breast cancer model. For this purpose, 4T1 tumor was injected to 46 Balb/c female mice and 30-40% hepatectomy was performed at the first (short term) and second weeks (long term) after injection. After sacrification of animals, CCL2, CCL3, CCL4, CCL5, CCL11, CCL17, CCL21, CCL22, CXCL1 and CXCL9 chemokine levels were evaluated. In addition, histopathological examination of resected liver tissues was performed. In animals with 4T1 tumor, levels of CCL2, CCL3, CCL4, CCL5, CCL22, CXCL1 and CXCL9 were found to be increased in short term hepatectomised mice, whereas; levels of CCL2, CCL3, CCL4, CCL5 and CXCL1 were found to be increased in long term hepatectomised mice. In total of 46 mice, 22 were injected with 4T1 tumor. In these 22 mice, only four had been found to have liver metastasis during histopathological examination. All of these four animals had been undergone hepatic resection. However, there was no statistically significant difference in histopathological analysis between sham and hepatectomy groups. In conclusion, there was an increase in chemokine levels after liver resection in this experimental breast cancer model. The metastatic effect of this condition has not been demonstrated in this study.