ANTİPARAZİTER İLAÇLAR YÜKLÜ HEDEFLENDİRİLMİŞ NANOTAŞIYICILARIN FORMÜLASYONU VE DEĞERLENDİRİLMESİ

Abstract

Gültekin, Y., Formulation and Evaluation of Targeted Nanocarriers Loaded with Antiparasitic Drugs, Hacettepe University Graduate School of Health Sciences, Pharmaceutical Technology Program, Ph.D Thesis, Ankara, 2024. Currently, a number of challenges encountered in the treatment of hydatid cyst disease restrict the treatment of hydatid cysts. The objective of this thesis was to evaluate the in vitro efficacy of nanocarriers loaded with albendazole sulfoxide and praziquantel and targeted with phytase enzyme on Echinococcus granulosus micro hydatid cysts as a new formulation. For this purpose, firstly, conjugation of PLGA polymer and phytase enzyme was carried out, then albendazole sulfoxide and praziquantel loaded nanoparticles were prepared using PLGA-Phytase polymer and characterization studies were carried out. Permeability and cytotoxicity tests were performed with cell culture studies of the formulations and It was found that the formulations did not exhibit any cytotoxic effects. In order to evaluate the efficacy of the prepared formulations, micro hydatid cysts were developed in vitro with Echinococcus granulosus protoscoleces taken from slaughterhouse animals and the prepared formulations were applied. The new formulation was found to be statistically more effective than the combination of free albendazole sulfoxide and praziquantel. Furthermore, ex vivo studies on the laminar layer of hydatid cysts obtained from sheep liver demonstrated that the new formulation binds to the laminar layer, increasing the permeability of albendazole sulfoxide and praziquantel through the laminar layer.