Besinsel Liflere Bağlı Biyoaktif Maddelerin Antioksidan Kapasitesi ve Rejenerasyon Davranışının Quencher Metoduyla Belirlenmesi

Abstract

This study aimed to investigate the regeneration potential of antioxidants bound to insoluble food matrix. Studies were performed in vitro with several food matrices rich in dietary fiber (DF) and bound antioxidants. After removing soluble fraction, antioxidant capacity (AC) of insoluble fraction was measured by the QUENCHER procedure using both ABTS+ and DPPH radical solutions. Then, this fraction was treated with either antioxidant solution or antioxidant-rich beverage under certain conditions to regenerate depleted antioxidants on the fiber, and its AC was measured again. Second and third regeneration steps were also performed. The results revealed that antioxidants bound to solid DF could be significantly regenerated up to three times by other antioxidant compounds present in the liquid phase. Regeneration efficiencies obtained on first regeneration step were found range between 21.5% and 154.3% depending on the type of insoluble food matrix and on the regeneration agent.