Sh-Sy5y Nöroblastoma Hücrelerinde Yüksek Glukozla Oluşturulan Hasara Karşı Neoeriositrinin Koruyucu Etkisinin Araştırılması
Özet
Diabetes mellitus is a common metabolic disease and its incidence increases dramatically.
With the spread of diabetes mellitus, there is a significant increase in the complications
of disease. Diabetic neuropathy is the most common disease among these complications.
Therefore, candidate natural agents have been searching for various medicines for
relieving, protecting or remediation against diabetic neuropathy. Flavonoids, which are
phenolic compounds found in plants, have antidiabetic and antioxidant effects.
Neoeriocitrin is a newly discovered flavonoid and there is not much information about its
effect. Therefore, this study has been planned by considering antioxidant effects of
flavonoids, neoeriocitrin may be a candidate agent. In this study, the protective effect of
neoeriocrine was investigated by causing damage to SH-SY5Y neuroblastoma cells with
high glucose concentration. In the study, crystal violet and trypan blue staining were
performed to determine cell viability. In order to determine its antioxidant properties, the
amount of intracellular ROS resulting from oxidative stress and the amount of MDA, iv
which is a lipid peroxidation marker, were measured. According to the results obtained,
it was found that neoeriocitrin decreased the amount of ROS at increasing concentrations
and had an effect on the reduction of lipid peroxidation at a concentration of 20 µM. On
the other hand, ATP levels were measured to determine the metabolic activity of the cell
and apoptotic cell death determined by the TUNEL test. According to the results, it was
found that the amount of ATP increased and the number of apoptotic cells decreased in
SH-SY5Y neuroblastoma cells at increasing concentrations of neoeriocitrin. Considering
all the results obtained from the study, it was revealed that neoeriocitrin had a protective
effect against the damage in SH-SY5Y cells that were modeled for diabetic neuropathy
with high glucose concentration.
