Neurotoxic Effects Of Bisphenol A On Sh-Sy5Y Neuroblastoma Cells Via Nitric Oxide

Abstract

Bisphenol A (BPA, 2,2-bis(4-hydroxyphenyl)propane)polycarbonate is an industrial component frequently used as an additive in the construction of epoxy resin and other non-polymer plastics. In this study cytotoxicity of low dose BPA (0.01 mu M-100 mu M) and NO levels in SH-SY5Y cells were assessed together to determine whether there is a correlation between of them. MTT Cytotoxicity assays were performed by applying BPA at increasing doses (0.01 mu M-100 mu M) for 8, 24, 72 hours. Measurements to determine the nitric oxide (NO) levels of the cells, were made using the Griess method at the same time intervals and at the equal doses with BPA. It is found that percentage viability values in SH-SY5Y cells treated with 100 JAM BPA for 8 hours, is reduced statistically significant compared to control groups (p<0.001). A significant correlation was found between cytotoxicity results and NO levels of cells treated BPA (0.01 mu M-100 mu M) for 8 and 72 hours ( p<0.01, p<0.001 respectively). Neurotoxic effects of low dose BPA exposure on SH-SY5Y cells may be explained with increase in the levels of NO as a result from different parameters. One of these parameters could be a disorder in the synthesis of nNOS on different stages such as transcription and translation.