Meme Kanser Hücrelerinde, Abce1 Ve Erf3 Proteinlerinin Sirna Taşıyan Nanoparçacıklarla İnhibe Edilme

Abstract

Nanoparticulate systems are the most important output of nanotechnology and can be used for diagnosis and treatment of many diseases. Chitosan nanoparticles have a widespread use in cancer treatments due to its properties such as biocompatibility, biodegradability and low toxicity. Novel tumor targeted therapies take places of surgery, chemotherapy and radiotherapy type conventional treatments. Targeted therapies preferred because of their specificity and minimal effect on healthy cells. These studies include drug loaded nanoparticulate systems which can help to the development of more effective cancer treatments. siRNA treatments which has an effect on an effect on mRNA level, are another novel technique for cancer treatment. The presented study proposed the application of siRNA loaded chitosan nanoparticles on cell lines to inhibit ABCE1 and eRF3 proteins for breast cancer treatment. In the first part of the study, chitosan polymers dissolved in acetic acid and crosslinked with TPP in a certaion pH to form nanoparticles. Particles prepared with ionic gelation method, characterized with zeta-sizer for size distribution and surface charge, then examined topologically with AFM and SEM to determine the desired nanoparticle forulations. Control, ABCE1 and eRF3 siRNA?s were loaded to chitosan nanoparticles. The affect of the siRNA loaded chitosan nanoparticles on breast cancer cell line (MCF-7) was observed in the second part of the study. A RTCA was used to determine the cell index of the nanoparticle applied cell lines. Moreover, WST-1 test was performed to determine the cell viability after the nanoparticle application. Fluorescent microscopy techniques were used determine whether the apoptotic or necrotic pathways were chosen by the cells after nanoparticle application. Results shows that, ABCE1 and eRF3 loaded chitosan particles dramatically reduces the proliferation of the breast cancer cells.