PRP Tatbikinin Timpanik Membran İyileşmesi Üzerine Etkisinin Rat Modeli Üzerinde İncelenmesi

Abstract

Platelet Rich Plasma (PRP) is a thrombocyte concentrate widely used in varios fields of medicine due to its wound healing enhancing properties. Its positive effects on wound healing and regeneration has been demonstrated in several studies. Owing to sparsity of the studies regarding this subject we intented to explore the outcomes of topical PRP application on the tympanic membrane healing. 10-12 weeks old, healthy, 24 Albino Wistar rats weighed 250-300 grams on average were used in this study. two ml of intracardiak blood was obtained following the anesthesia. PRP acquired by the way of centrifugation from the obtained blood. Subtotal perforation was created posterior to malleus in both ears under the surgical microscope. %10 Silver Nitrate used to process the margins of the perforation. After the gelfoams were submerged in the obtained PRP for five minutes, left ear perforations were repaired in the hourglass fashion. For the right ears in control group, gelfoams were submerged in physiological saline for five minutes before the perforations repaired in similar fashion. Animals were subjected to microscopic examination with regular intervals following the procedure and the infection was observed in none. Subjects were sacrified in 2. and 4. weeks followed by the extraction and hystopathological examination of specimens. Duration for the closure of the tympanic membran was assessed to be eight and nine days on average in study and control groups respectively. This difference was found to be significant following statistical comparison(p=0.01). Closure rate for the perforated tympanic membrane was found to be 21 (%87.5) and 15 (%62.5) in study and control groups respectively with difference being insignificant following statistical comparison(p=0.07). Healing rates of lamina propria assessed by hystopathological examination were 19 (%92.9) and nine (%57.1) in study and control groups respectively with difference being insignificant following statistical comparison(p=0.063). Though difference being insignificant following statistical comparison, the closure rate of the perforated tympanic membrane, and healing rate of lamina propria observed to be better in PRP utilized study group. Considering the reports from literature and findings of our study usage of PRP can be recommended for its effectiveness in wound healing, lack of evident side effects, and low-cost in patient population beset with impaired wound healing due to various reasons and additional diseases.