Nanoteknolojik Yaklaşımlarla Koagülatif Sistemlerin Geliştirilmesi
Özet
Uncontrolled bleeding is the major cause of death in cases like war and disaster in
addition of bleeding dependant trauma encountered in daily life. Effective bleeding
control is essential because a lot of injured patients or limited medical equipment
on battlefield. Currently for bleeding control was used such as bandage, direct
pressure, tourniquet or cauterization. But these conventional techniques remain
incapable or not used in much injury. Therefore hemostatic agent requirement,
that is portable, low cost, no adverse effects to healing, effective control of
bleeding, easy administration even by layperson or by first-aider, increases day by
day. When the conditions and environmental factors play a role in hemostasis
were considered, products produced by nanotechnological approaches will
iv
facilitate obviously the hemostasis. Similarity of the nanofibrilar structure to
collagen/elastin fibers in extracellular matrix and its high surface area/volume ratio
will accelerate thrombocyte migration in structure.
Within the scope of the thesis; it’s planned that the double layer blood stopping
patches are prepared. At first step, top layer of hemostatic patches are prepared
from cellulose and chitosan that is frequently used in medical industry. Thereafter,
hemostatic agents (calcium ions, vitamin K, kaolin etc.) that plays active role in
coagulation cascade are incorporated the structure of the hemostatic patches. A
nanofibrillar layer membrane on the top is created from the silk fibroin that widely
used as a suture material and phosphatdylcholine that be in the structure of cell.
The performances of the prepared modified silk fibroin nanofibrillar patches are
investigated comparatively. Surface properties of hemostatic dressing are
characterized using Scanning Electron Microscopy (SEM). The activity of the
prepared hemostatic dressings is examined by in-vitro and in-vivo coagulation
tests. The coagulation time of blood is determined using by partial thromboplastin
time (aPTT) and protrombin time (PTZ) tests. The amount of adhered thrombocyte
on the hemostatic dressings is compared using LDH assay. In vivo study, the
hemostatic activity of the hemostatic dressings is tested in healty and diabetic rat femoral artery model.