Meme Kanseri Tedavisinde Paklitaksel Yüklü Polikatyonik ve Anyonik Siklodekstrin Nanoküre Formülasyonu ve in Vitro Değerlendirilmesi

Abstract

Paclitaxel (PCX) is a well-known anti-cancer agent that has poor water solubility and poor stability displaying cytotoxic activity against breast cancer. The commercial formulation contains Cremophor® EL. However this vehicle causes severe side effects such as neurotoxicity and neuropathy. Amphiphilic cyclodextrins have the ability to form self-organizing nanoparticles spontaneously. Due to their properties they have been used for the development of drug delivery systems in nanoscale. The aim of this study was to determine the effect of in vitro properties of amphiphilic CD nanospheres with different surface charges on cellular interaction and drug loading capacity as novel polycationic ßCDC6 nanoparticle excipient, safety and efficacy and encapsulation of PCX stable physically. Negatively charged 6-O-CAPRO-ß-CD, pozitively charged chitosan coated 6-O-CAPRO-ß-CD and polycationic ßCDC6 nanospheres were formulated. Mean particle diameter, zeta potential, drug loading and in vitro drug release profiles were determined and compared with each other. According to different parameters polycationic ß-CDC6 nanospheres were prepared and in vitro characterization was performed. With the appropriate mean particle size(<200nm) different surface charged nanospheres were observed. Blank nanoparticles maintained their stability for 12 months. Positively charged nanoparticles displayed higher drug loading capacity. PCX loaded nanospheres maintained their stability for 30 days. Blank nanospheres have no cytotoxic effect against L-929 cells. In addition, PCX loaded nanospheres have higher anticancer effect when compared with the PCX solution against MCF-7 cells.