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dc.contributor.authorAlonso-Ferrero, Maria E.
dc.contributor.authorvan Til, Niek P.
dc.contributor.authorBartolovic, Kerol
dc.contributor.authorMata, Márcia F.
dc.contributor.authorWagemaker, Gerard
dc.contributor.authorMoulding, Dale
dc.contributor.authorWilliams, David A.
dc.contributor.authorKinnon, Christine
dc.contributor.authorWaddington, Simon N.
dc.contributor.authorMilsom, Michael D.
dc.contributor.authorHowe, Steven J.
dc.date.accessioned2020-01-17T10:15:57Z
dc.date.available2020-01-17T10:15:57Z
dc.date.issued2018
dc.identifier.issn0301-472X
dc.identifier.urihttps://doi.org/10.1016/j.exphem.2017.09.003
dc.identifier.urihttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5731634/
dc.identifier.urihttp://hdl.handle.net/11655/21793
dc.description.abstractIntegration-deficient lentiviruses (IdLVs) deliver genes effectively to tissues but are lost rapidly from dividing cells. This property can be harnessed to express transgenes transiently to manipulate cell biology. Here, we demonstrate the utility of short-term gene expression to improve functional potency of hematopoietic stem and progenitor cells (HSPCs) during transplantation by delivering HOXB4 and Angptl3 using IdLVs to enhance the engraftment of HSPCs. Constitutive overexpression of either of these genes is likely to be undesirable, but the transient nature of IdLVs reduces this risk and those associated with unsolicited gene expression in daughter cells. Transient expression led to increased multilineage hematopoietic engraftment in in vivo competitive repopulation assays without the side effects reported in constitutive overexpression models. Adult stem cell fate has not been programmed previously using IdLVs, but we demonstrate that these transient gene expression tools can produce clinically relevant alterations or be applied to investigate basic biology.tr_TR
dc.language.isoentr_TR
dc.publisherElsevier Science Bvtr_TR
dc.relation.isversionof10.1016/j.exphem.2017.09.003tr_TR
dc.rightsinfo:eu-repo/semantics/openAccesstr_TR
dc.subjectHSPCstr_TR
dc.subjectHOXB4tr_TR
dc.subjectIdLVstr_TR
dc.subjectbasic biologytr_TR
dc.subject.lcshKök Hücretr_TR
dc.titleEnhancement of Mouse Hematopoietic Stem/Progenitor Cell Function Via Transient Gene Delivery Using Integration-Deficient Lentiviral Vectorstr_TR
dc.typeinfo:eu-repo/semantics/articletr_TR
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.relation.journalExperimental Hematologytr_TR
dc.contributor.departmentKök Hücretr_TR
dc.identifier.volume57tr_TR
dc.identifier.startpage21tr_TR
dc.identifier.endpage29tr_TR
dc.description.indexWoStr_TR
dc.description.indexScopustr_TR
dc.description.indexPubMedtr_TR
dc.fundingYoktr_TR


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