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dc.contributor.authorDemiralp, B
dc.contributor.authorChen, HL
dc.contributor.authorKoh, AJ
dc.contributor.authorKeller, ET
dc.contributor.authorMcCauley, LK
dc.date.accessioned2019-12-16T06:53:41Z
dc.date.available2019-12-16T06:53:41Z
dc.date.issued2002
dc.identifier.issn0013-7227
dc.identifier.urihttps://doi.org/10.1210/en.2002-220221
dc.identifier.urihttp://hdl.handle.net/11655/19146
dc.description.abstractPTH has anabolic and catabolic actions in bone that are not clearly understood. The protooncogene c-fos and other activating protein 1 family members are critical transcriptional mediators in bone, and c-fos is up-regulated by PTH. The purpose of this study was to examine the mechanisms of PTH and the role of c-fos in PTH-mediated anabolic actions in bone. Mice with ablation of c-fos (-/-) and their wild-type (+/+) and heterozygous (+/-) littermates were administered PTH for 17 d. The +/+ mice had increased femoral bone mineral density (BMD), whereas -/- mice had reduced BMD after PTH treatment. PTH increased the ash weight of +/+ and but not -/-, femurs and decreased the calcium content of but not +/+ or +/-, femurs. Histomorphometric analysis showed that PTH increased trabecular bone volume in c-fos +/+, +/- vertebrae, but, in contrast, decreased trabecular bone in -/- vertebrae. Serum calcium levels in +/+ mice were greater than those in -/- mice, and PTH increased calcium in -/- mice. Histologically, PTH resulted in an exacerbation of the already widened growth plate and zone of hypertrophic chondrocytes but not the proliferating zone in -/- mice. PTH also increased calvarial thickness in +/+ mice, but not -/- mice. The c-fos -/- mice had lower bone sialoprotein and osteocalcin (OCN), but unaltered PTH-1 receptor mRNA expression in calvaria, suggesting an alteration in extracellular matrix. Acute PTH injection (8 h) resulted in a decrease in osteocalcin mRNA expression in wild-type, but unaltered expression in -/-, calvaria. These data indicate that c-fos plays a critical role in the anabolic actions of PTH during endochondral bone growth.
dc.language.isoen
dc.publisherEndocrine Soc
dc.relation.isversionof10.1210/en.2002-220221
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectEndocrinology & Metabolism
dc.titleAnabolic Actions of Parathyroid Hormone during Bone Growth Are Dependent on C-Fos
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.relation.journalEndocrinology
dc.contributor.departmentPeriodontoloji
dc.identifier.volume143
dc.identifier.issue10
dc.identifier.startpage4038
dc.identifier.endpage4047
dc.description.indexWoS
dc.description.indexScopus


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