Prostat Kanser Tayini İçin Moleküler Baskılama Temelli Yüzey Plazmon Rezonans (Spr) Sensörlerin Hazırlanması

Abstract

Surface plasmon resonance (SPR) biosensors are used to characterize and quantify biomolecular interactions. Real-time and fast measurement, high sensitivity and specificity, no need of labeled reagents are the unique properties of SPR biosensors. SPR biosensors have been used for detection of several analytes. Recently, molecular imprinting technology is used for creation of biorecognition surfaces on the SPR biosensors. Molecular imprinting is the technology of designing materials with robust recognition sites suitable for interaction with the print molecule. Due to their stability in complex matrices under tough conditions, ease of preparation, low cost, reusability, high recognition capacity to the template molecule and binding characteristics as high as those of natural binders, molecularly imprinted polymers (MIPs) are extensively used in a variety of areas, such as catalysis, separations, solid-phase extractions and biosensors. In this study, an SPR biosensor was prepared for prostate specific antigen (PSA) detection. PSA was imprinted onto the modified SPR sensor surface via microcontact imprinting. Detailed characterization studies of PSA imprinted (PSAMIP) SPR sensors were performed by observation using atomic force microscope (AFM), ellipsometry, scanning electron microscope (SEM), Raman, FT-IR and iv

contact angle measurements. PSA detection studies were performed by using standard PSA solutions. Selectivity of PSA-MIP SPR sensors against PSA was tested by using human serum albumin (HSA), immunoglobulin G (IgG) and lysozyme as competitive proteins. Kinetics and isotherm parameters were calculated by applying association kinetics analysis. Furthermore, PSA detection was also carried out from PSA-spiked human serum samples and prostate cancer patients’ serum samples. Recovery % was calculated and to determine the accuracy and reliability of the developed method, results were compared with the ELISA method which is currently used in central laboratories and hospitals for PSA detection. In the last step, reusability of the PSA-MIP SPR sensors was tested. The results obtained from the study show that the developed PSA-MIP sensor could be used as an alternative and superior method to ELISA with its unique properties that must be in a well qualified biosensor.