Plastik Ve Doğal Balmumu Yüksüklerde Üretilen Arı Sütlerinin Mikrobiyal Yüklerinin, Protein İçeriklerinin Ve Antimikrobiyal Etkinliklerinin Araştırılması

Abstract

In recent years, interest in honey bee and bee products has increased day by day due to the increase in the level of welfare, accelerating scientific studies on beekeeping, and high income return. These developments have made apitherapy which means treatment with bee products very popular. Besides bee products such as honey, propolis, pollen, bee stew, honey wax, studies on royal jelly have gained great importance as it plays a decisive role in the life of the colony as well as its effectiveness in nutrition and strong antimicrobial activity in human health. In this thesis, comparative experiments were carried out to determine whether royal jelly obtained from natural wax grafted by the ‘’doolittle method’’ used for larval transfer in traditional beekeeping and plastic queen cell cups is different in terms of microbial content, antimicrobial activity and protein concentrations. In the scope of the study, NA, BHI, SDA , Endoagar and Milkagar were done 3 times for the microbial content examination by the inoculating spreading method of royal jelly produced by natural and plastic queen cell cups. After incubation at 37° iv

C for 24 hours, no growth was observed in the petri dishes and no microbial content was observed in the royal jelly. Zone diameters generated by royal jelly on microorganisms which were sown by spreading to Nutrient Agar and Saboruod Dextrose Agar method were measured after 24 hours to test for antimicrobial activity by disk diffusion experiment method. As a result of the diameter measurements, it was found that the efficiency of royal jelly produced in both types of fermentation is as strong as OTC when compared with OTC used as a positive control. In addition, the zone diameters of royal jelly were developed at the acute moment, and after a while due to the intense protein content of the royal jelly, secondary zone diameters were observed as microorganisms which were inoculated used it as nutrient media. The Lowry method was used to determine the protein content of the obtained royal jelly. It was found that royal jelly obtained from natural and plastic queen cell cups was not different in terms of protein concentrations. At the end of the study, royal jelly obtained from natural wax and plastic queen cell cups did not differ in terms of examined parameters; the result is that plastic queen cell cups commonly used in royal jelly production do not cause any inconveniences when used in the larval transfer process.