Yüzeyde Güçlendirilmiş Raman Spektroskopisi Tekniği Kullanılarak Hedef Dna Dizi Tayini

Abstract

Within the scope of this study, development of a sensitive deoxyribonucleic acid (DNA) biosensor by using surface enhance Raman spectroscopy (SERS) technique was studied. For this purpose two kinds of magnetic nanoparticles (Gold coated magnetic sphere and silica coated 3-aminopropyltriethoxysilane (APTS) modified magnetic nanoparticle) were used. Besides all these, gold nanorods which were become SERS active by coating with one of the SERS label molecule 5,5-dithiobis (2-Nitrobenzoic acid) (DTNB) and thus enables the detection of target sequences were used. In the first step of the study, all of the parameters in each step were optimized to ensure proper surface modifications for the nanoparticles and get the most out of the edited system. After that a system that is called ‘sandwich assay’ was applied for rapid, selective and sensitive detection of genetic modified organisms (GMO). According to the system magnetic particles coated with target specific probes were enabled selective seperation of the target sequence from the media and DTNB coated nanorods made it possible to detect target sequence by following the symmetric NO2 stretching band that incident to DTNB molecule. To obtain calibration curve from the system that developed for GMO analysis, it was studied between 50- 1000 nM target sequence molarities and R2 between the band intensities was 0,955.