İlaç Salımı için Mitomisin C Baskılanmış Manyetik Partikül Gömülü Kriyojellerin Hazırlanması

Abstract

Controlled drug release is a technique used for releasing the drug in the desired site of the body at the therapeutic dose. In drug release systems, administration of the drug given to the patient in appropriate doses to reduce the side effects of the drug is the main goal of drug release systems. Targeted drug transport can be accomplished using magnetic polymers within the presence (or by the orientation) of the magnetic field, which is considered as a new approach between drug release systems. In this approach, after the magnetic polymers are administered intravenously to the patient, the magnetic particles are directed to the target area by applying an external magnetic field to the body and it is aimed to perform drug release only in that area. In topical applications, the amount of drug released and the speed of release can be controlled easily by applying a magnetic field to the desired area from outside of the body. Thus, while the therapeutic effectiveness of the drug is increased, the side effects of the drug on healthy cells and unimpaired tissues are also minimized. Mitomycin C is a broad-spectrum antibiotic. Mitomycin C is a DNA alkylating agent widely used in chemotherapy treatments against cancer due to its non-selective antitumor activity. Mitomycin C is an anti-cancer drug that can be obtained from the culture of the bacteria named Streptomyces caespitosus, used alone or in combination with other chemotherapeutic agents and in gastrointestinal, lung, cervical, head-neck, pancreatic and bladder cancers, and chronic leukemia disease. Mitomycin C displays an antitumor characteristic by inhibiting DNA replication in tumor cells via creating cross-links between the two separate chains of DNA double helix structure. There are many acute and chronic toxic properties which limit the use of Mitomycin C in clinical practice. Various release methods have been developed in the literature to reduce the toxic effects of Mitomycin C. One of the most interesting and effective approaches to drug release in recent years is the molecular imprinting method. With the molecular imprinting technique, three-dimensional nano-cavities are created in the drug-carrier structure suitable for the target molecule. Both inorganic and polymer-based drug carrier systems can be used for the molecular imprinting technique. Among polymer-based carriers, cryogels have been a focus of interest popularly in recent years. Cryogels are prepared at low temperatures with partially frozen monomer or polymer solutions. Their preparation at low temperatures completely eliminates the possibility of drug degradation in the structure. This is a very important advantage maintained by cryogels. Being able to adjust the pore size easily is also very important to control the rate of drug release. Their highly hydrophilic structure provides a great biocompatibility. In this thesis study, a new composite drug carrier system was prepared by combining both magnetic properties of magnetic polymeric particles and advantages of cryogel structure. In the first part of the thesis, magnetic microparticle embedded composite cryogel drug release system was prepared and structural characterization studies were carried out. In the second part of the study, model drug Mitomycin C imprinting and release performances were examined in-vitro.