dc.contributor.author | Winklmeier, Stephan | |
dc.contributor.author | Schlüter, Miriam | |
dc.contributor.author | Spadaro, Melania | |
dc.contributor.author | Thaler, Franziska S. | |
dc.contributor.author | Vural, Atay | |
dc.contributor.author | Gerhards, Ramona | |
dc.contributor.author | Macrini, Caterina | |
dc.contributor.author | Mader, Simone | |
dc.contributor.author | Kurne, Aslı | |
dc.contributor.author | Inan, Berin | |
dc.contributor.author | Karabudak, Rana | |
dc.contributor.author | Özbay, Feyza Gül | |
dc.contributor.author | Esendagli, Gunes | |
dc.contributor.author | Hohlfeld, Reinhard | |
dc.contributor.author | Kümpfel, Tania | |
dc.contributor.author | Meinl, Edgar | |
dc.date.accessioned | 2021-06-03T05:43:00Z | |
dc.date.available | 2021-06-03T05:43:00Z | |
dc.date.issued | 2019 | |
dc.identifier.issn | 2332-7812 | |
dc.identifier.uri | http://dx.doi.org/10.1212/NXI.0000000000000625 | |
dc.identifier.uri | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6857907/ | |
dc.identifier.uri | http://hdl.handle.net/11655/24128 | |
dc.description.abstract | Objective To identify circulating myelin oligodendrocyte glycoprotein (MOG)-specific B cells in the blood of patients with MOG antibodies (Abs) and to determine whether circulating MOG-specific B cells are linked to levels and epitope specificity of serum anti-MOG-Abs. Methods We compared peripheral blood from 21 patients with MOG-Abs and 26 controls for the presence of MOG-specific B cells. We differentiated blood-derived B cells in vitro in separate culture wells to Ab-producing cells via engagement of Toll-like receptors 7 and 8. We quantified the anti-MOG reactivity with a live cell–based assay by flow cytometry. We determined the recognition of MOG epitopes with a panel of mutated variants of MOG. Results MOG-Ab–positive patients had a higher frequency of MOG-specific B cells in blood than controls, but MOG-specific B cells were only detected in about 60% of these patients. MOG-specific B cells in blood showed no correlation with anti-MOG Ab levels in serum, neither in the whole group nor in the untreated patients. Epitope analysis of MOG-Abs secreted from MOG-specific B cells cultured in different wells revealed an intraindividual heterogeneity of the anti-MOG autoimmunity. Conclusions This study shows that patients with MOG-Abs greatly differ in the abundance of circulating MOG-specific B cells, which are not linked to levels of MOG-Abs in serum suggesting different sources of MOG-Abs. Identification of MOG-specific B cells in blood could be of future relevance for selecting patients with MOG-Abs for B cell–directed therapy. | |
dc.language.iso | en | |
dc.relation.isversionof | 10.1212/NXI.0000000000000625 | |
dc.rights | Attribution 4.0 United States | |
dc.rights | info:eu-repo/semantics/openAccess | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.title | Identification Of Circulating Mog-Specific B Cells In Patients With Mog Antibodies | |
dc.type | info:eu-repo/semantics/article | |
dc.type | info:eu-repo/semantics/publishedVersion | |
dc.relation.journal | Neurology® Neuroimmunology & Neuroinflammation | |
dc.contributor.department | Nöroloji | |
dc.identifier.volume | 6 | |
dc.identifier.issue | 6 | |
dc.description.index | PubMed | |
dc.description.index | WoS | |
dc.description.index | Scopus | |