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dc.contributor.authorHançerlioğulları, Begüm Zeynep
dc.contributor.authorKöksel, Hamit
dc.contributor.authorDudak, Fahriye Ceyda
dc.date.accessioned2021-03-23T08:37:08Z
dc.date.available2021-03-23T08:37:08Z
dc.date.issued2018
dc.identifier.urihttps://onlinelibrary.wiley.com/doi/full/10.1002/jsfa.9113
dc.identifier.urihttps://pubmed.ncbi.nlm.nih.gov/29736935/
dc.identifier.urihttp://hdl.handle.net/11655/23600
dc.identifier.urihttps://onlinelibrary.wiley.com/doi/epdf/10.1002/jsfa.9113
dc.description.abstractBACKGROUND: Since the common protease substrates did not give satisfactory results for the determination of Sunn pestprotease activity in damaged wheat, different peptide substrates derived from the repeated sequences of high molecular weightglutenin subunits were synthesized.RESULTS: Hydrolysis of peptides by pest protease was determined by high-performance liquid chromatography. Among threepeptides having the same consensus motifs, peptide1 (PGQGQQGYYPTSPQQ) showed the best catalytic efficiency. A novel assaywas described for monitoring the enzymatic activity of protease extracted from damaged wheat flour. The selected peptide waslabeled with a fluorophore (EDANS) and quencher (Dabcyl) to display fluorescence resonance energy transfer. The proteolyticactivity was measured by the change in fluorescence intensity that occurred when the protease cleaved the peptide substrate.Furthermore, the assay developed was modified for rapid and easy detection of bug damage in flour. Flour samples weresuspended in water and mixed with fluorescence peptide substrate. After centrifugation, the fluorescence intensities of thesupernatants, which are proportional to the protease content of the flour, were determined.CONCLUSION: The total analysis time for the assay developed is estimated as 15 min. The assay developed permits a significantdecrease in time and labor, offering sensitive detection of Sunn pest damage in wheat flour.tr_TR
dc.language.isoentr_TR
dc.publisherJohn Wileytr_TR
dc.relation.isversionof10.1002/jsfa.9113tr_TR
dc.rightsinfo:eu-repo/semantics/openAccesstr_TR
dc.subjectSunn pesttr_TR
dc.subjectWheattr_TR
dc.subjectPeptide substratetr_TR
dc.subjectFRETtr_TR
dc.subject.lcshGıda mühendisliğitr_TR
dc.titleDevelopment of a peptide substrate for detection of sunn pest damage in wheat flourtr_TR
dc.typeinfo:eu-repo/semantics/articletr_TR
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.relation.journalJournal of the Science of Food and Agriculturetr_TR
dc.contributor.departmentGıda Mühendisliğitr_TR
dc.identifier.volume98tr_TR
dc.identifier.issue15tr_TR
dc.identifier.startpage5677tr_TR
dc.identifier.endpage5682tr_TR
dc.description.indexWoStr_TR
dc.description.indexPubMedtr_TR
dc.fundingTÜBİTAKtr_TR


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