Doku Mühendisliği Uygulamalarında Kullanılmak Üzere Peptid Bazlı Nano Ölçekli Doku Iskelelerinin Hazırlanması Ve Karakterizasyonu

Abstract

Tissue scaffolds made of various biomaterials such as PEG, PLLA, PGA, PHEMA or chitosan have being used for different purposes lately. In recent years, studies have been focused especially on three-dimensional cell culture, so for tissue scaffolds created, scientists try to mimic ECM. Altough synthetic 3D systems which have been created helped a lot to understand many cellular activity, because the diameter of fibers are almost the same as the cells, cells behave to be in a 2D culture. Alternately used, biomaterials developed from animals may overcome the problem of diameter, but because they contain residual growth factors and are not suitable for human studies as well as being not pure enough, completely controlled studies can not be performed. Therefore, self assembling peptide scaffolds started to be used. Self assembling of natural or synthetic materials are promising for many applications. In this study, self-assembling Fmoc-FF-OH dipeptides dissolved in the cell media have self organized to form hydrogel scaffolds. Then optimization studies performed with Fmoc-FF-OH hydrogels both containing nano-hydroxyapatite and empty, and characterizations are done. 3D culture of chondrocytes in Fmoc-FF-OH scaffolds has also been performed, in which cells were encapsulated, and histological experiments are performed.