Yüzey Baskılama Yöntemiyle Seçici Ardorbentlerin Hazırlanması, Karakterizasyonu ve Kromatografik Uygulamaları

Abstract

Today, surface imprinted polymers emerge as synthetic adsorbents with application in several areas. In this study, adsorptions of Cu(II) ion and model protein (lysozyme) from aqueous solution were investigated using Cu(II) ion and lysozyme imprinted poly(HEMA-GMA) cryogels via batch wise experiments. Polyethyleneimine (PEI) was used as ligand for the polymeric structure. Binding of Cu(II) ions into the structure was achieved via electrostatic interactions and adsorption of lysozyme was performed under favor of Cu(II) ions bounded to the structure. Characterization of poly(HEMA-GMA) cryogels was conducted via Fourier transformed infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and microcomputerized tomography (μCT). The amount of Cu(II) ions before and after adsorption process was estimated using atomic absorption spectroscopy (AAS) whereas that of lysozyme molecules was determined using UV-visible spectroscopy.